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Key allosteric residues are predicted based on sequence coevolution analysis, which serves as guidelines for allosteric drug design and optimization.

Reengineering the nucleotide-binding pocket of an extant ATPase to restore ancestral GTPase activity revealed an ATP-dependent intermediate required for function and suggested why the protein evolved to use ATP.

Co-aggregation, amyloid formation, and unidirectional cross-seeding of prolactin and galanin for their efficient storage in secretory granules of pituitary and subsequent functional hormone release.

Fly protein families Dprs and DIPs can create a multitude of complementary interfaces for homo- and heterophilic adhesion complexes, resulting in instructive roles for connectivity in the motor neuron circuitry.

A straightforward, rapid and generalizable strategy uses modified nanobodies to convert intracellular protein detection into desired biological activities in cells and animals.

Antibody paratopes can be added to the human repertoire by CRISPR-Cas9 editing of B lymphocytes using a strategy that allows for hypermutation and class switching of resulting B cell receptors.

Orthologous proteins display different mutational robustness that can be leveraged to improve prediction of functional sequences.

A new method for developing biosensors from ligand binding domains that have concentration-dependent and selective responses to small molecules in yeast, mammalian cells and plants.

An optimized CRISPR-Cas9 system enables multiplexed genome engineering for evolving biomolecules and pathways from chromosomally integrated DNA libraries.

Combining live imaging and advanced electron microscopy gives insights into the structural integrity of the nematode epidermis and its intimate connection with the extracellular matrix.