4,302 results found
    1. Genetics and Genomics

    Generation of a versatile BiFC ORFeome library for analyzing protein–protein interactions in live Drosophila

    Johannes Bischof et al.
    Generation of a new fly line library for analysing protein–protein interactions in vivo.
    1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    Transient protein-protein interactions perturb E. coli metabolome and cause gene dosage toxicity

    Sanchari Bhattacharyya et al.
    Weak yet highly species-specific protein-protein interactions enhance the activity of metabolically related enzymes in bacteria at endogenous conditions, but also mean that overexpression of one partner leads to permanent non-physiological complexes and gene dosage toxicity.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Single methyl groups can act as toggle switches to specify transmembrane Protein-protein interactions

    Li He et al.
    The placement of single methyl groups at certain positions in the sequence of small model transmembrane proteins consisting solely of leucines and isoleucines can modulate highly specific, productive interactions with the transmembrane domain of the erythropoietin receptor.
    1. Cell Biology

    Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-protein interactions

    Christina K Kim et al.
    SPARK2 allows a transcriptional readout of inter- and intracellular protein-protein interactions, with near-zero background, by employing proximity-dependent luciferase-LOV regulation.
    1. Cancer Biology

    BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions

    Nicolas Bery et al.
    A biosensor resource has been developed to monitor the intracellular interactions of RAS family proteins with effector molecules and the effects of inhibitors as an aid to drug development.
    1. Structural Biology and Molecular Biophysics

    Monochromatic multicomponent fluorescence sedimentation velocity for the study of high-affinity protein interactions

    Huaying Zhao et al.
    Fluorescence detected sedimentation velocity offers a new method for studying heterogeneous protein interactions in solution by exploiting characteristic temporal signal modulations of photoswitchable fluorescent proteins.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Single-molecule force spectroscopy of protein-membrane interactions

    Lu Ma et al.
    A high-resolution method to quantify interactions between lipid bilayers and single proteins under controlled load is presented and applied to key proteins involved in membrane fusion and formation and maintenance of membrane contact sites.
    1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    Emergence and evolution of an interaction between intrinsically disordered proteins

    Greta Hultqvist et al.
    The resurrection of ancient ancestral intrinsically disordered proteins reveals the evolution of a protein-protein interaction in molecular detail.
    1. Structural Biology and Molecular Biophysics

    Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states

    Dan Tan et al.
    An enrichable cross-linker with optional isotope labeling quadruples the number of cross-linked peptides identified from high-complexity samples, enhancing proteome-wide analysis of protein-protein interactions and protein conformational changes by mass spectrometry.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells

    Chitra Mohan et al.
    An in vivo disulfide crosslinking assay shows preferential disassembly of nucleosomes with two H2A.Z histones by transcription machinery in yeast and conjugation to one or two ubiquitin moieties in human cells.

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