ATF4 is a metabolic effector of mTORC1 signaling, co-opted to induce gene targets involved in amino acid synthesis, uptake, and tRNA charging, contributing to mTORC1-driven protein and glutathione synthesis.
Synthetic single domain antibody libraries and a binder selection cascade encompassing ribosome and phage display enable the selection of conformation-specific binders against previously intractable membrane proteins within three weeks.
The development of a transgenic zebrafish allows for cell-type-specific labeling of neuronal protein synthesis, enabling brain-wide visualization and quantification of protein synthesis and demonstrating region-specific increases following elevated brain activity.
Mitochondria can tune the protein synthesis of nuclear-encoded proteins through condition-dependent mRNA localization that is regulated by translation elongation and the geometric constraints of the cell.
Behavioral and synaptic investigations of long-term memory in Aplysia reveal differing roles for DNA methylation, protein synthesis during training and protein synthesis shortly after training with respect to memory consolidation and maintenance.
As the function of membrane proteins is often influenced by its membrane environment the presented combinatorial approach is of great value for the investigation of membrane protein complexes in natural or even manipulated lipid bilayers.