Kristian Parey, Ulrich Brandt ... Volker Zickermann
The site of ubiquinone binding observed in the cryo-EM structure of respiratory complex I during turnover supports a two-state stabilization change mechanism.
By performing 15N pulse-labeling of mice, the turnover of hundreds of proteins in eye tissues was measured by mass spectrometry that revealed long-lived metabolic enzymes in the lens.
The analysis of mass-spectrometry data for all possible open reading frames reveals protein evidence for evolutionarily young, unannotated proteins with distinct characters.
Sheila Hoffmann-Conaway, Marisa M Brockmann ... Carolina Montenegro-Venegas
The presynaptic scaffolding protein Bassoon is involved in regulating neurotransmitter release by controlling synaptic vesicle pool size and vesicular protein turnover through increased ubiquitination and Parkin-dependent autophagy.
Intracellular protein gradients, which are formed by the combined action of cytoplasmic diffusion and cortical transport toward the cell pole, are solely determined by cell shape.
Paul Fischer, Shatanik Mukherjee ... Peter Hegemann
UV-Vis- and IR-spectroscopy provides insights into the light-induced enzyme activity of a rhodopsin guanylyl cyclase by tracking the substrate turnover with atomic resolution in real-time and correlating it with the structural changes of the protein.
Structures of CysZ show a antiparallel membrane protein with an unanticipated fold and together with functional characterization provide insight into a bacterial sulfate translocating system.
David J Turner, Alexander Saveliev ... Martin Turner
A genetic screening approach identifies RNA binding proteins that enhance or inhibit the accumulation of CD138+ plasma cells in vitro, and chimeric mouse models demonstrate that YTHDF2 promotes the accumulation of antibody secreting cells in vivo.
