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    1. Structural Biology and Molecular Biophysics

    Structural dynamics of E. coli single-stranded DNA binding protein reveal DNA wrapping and unwrapping pathways

    Sukrit Suksombat et al.
    Single-molecule force and fluorescence spectroscopy reveal the structural states and dynamics of E. coli single-stranded DNA binding proteins and the energy landscape of the nucleo–protein complex.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    The Cas4-Cas1-Cas2 complex mediates precise prespacer processing during CRISPR adaptation

    Hayun Lee et al.
    During CRISPR adaptation, Cas4 forms a ternary complex with the Cas1-Cas2 spacer integration complex, an interaction that coordinates substrate hand-off following precise, PAM-dependent prespacer processing prior to integration.
    1. Computational and Systems Biology

    A network of epigenetic modifiers and DNA repair genes controls tissue-specific copy number alteration preference

    Dina Cramer et al.
    The genetic background of a cancer cell and the chromatin organization of the tissue-of-origin impact the amount, length and position of somatic copy number alterations in cancer.
    1. Cell Biology

    In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates

    Weirui Ma et al.
    Binding of a multivalent RNA-binding protein to mRNAs that are able to form pervasive RNA–RNA interactions induces formation of mesh-like condensates, whereas binding of mostly structured mRNAs induces sphere-like condensates.
    1. Biochemistry and Chemical Biology

    Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition

    Clare Rollie et al.
    The acquisition of new foreign DNA by the prokaryotic adaptive immune system CRISPR-Cas is shown to depend on the fundamental sequence specificity of the Cas1 integrase.
    1. Evolutionary Biology

    Intermolecular epistasis shaped the function and evolution of an ancient transcription factor and its DNA binding sites

    Dave W Anderson et al.
    Experimental mapping of the joint sequence space of an ancient transcription factor (TF) and its DNA binding sites reveals that epistasis across the molecular interface permitted the evolution of a new and specific TF-DNA complex.
    1. Structural Biology and Molecular Biophysics

    Structural basis for recognition and remodeling of the TBP:DNA:NC2 complex by Mot1

    Agata Butryn et al.
    A structural analysis of the transcription regulator Mot1 in complex with promoter DNA and the proteins TBP and NC2 provides a first structural framework for how a Swi2/Snf2 type remodeler interacts with a histone fold protein:DNA complex.
    1. Biochemistry and Chemical Biology

    How to measure and evaluate binding affinities

    Inga Jarmoskaite et al.
    Simple guidelines and a checklist are provided for performing high-quality equilibrium binding measurements.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    A novel motif of Rad51 serves as an interaction hub for recombination auxiliary factors

    Negar Afshar et al.
    Although Rad51 is the central protein involved in recombinational DNA repair, multiple auxiliary factors potentiate its activity by binding to a single, evolutionarily conserved motif.
    1. Structural Biology and Molecular Biophysics
    2. Chromosomes and Gene Expression

    The Cac1 subunit of histone chaperone CAF-1 organizes CAF-1-H3/H4 architecture and tetramerizes histones

    Wallace H Liu et al.
    Biophysical and biochemical approaches reveal new insights into the architecture of CAF-1 and the unique mechanism by which CAF-1 tetramerizes histones H3/H4.