Measurement of post-translational modifications in primary macrophages infected with Mtb revealed phosphorylation of TAX1BP1, an autophagy receptor that enables full maturation of the Mtb autophagosome.
Combined light and electron microscopy reveals a new function for Arp2/3-mediated actin assembly in nuclear envelope rupture, which leads to a separation of nuclear membranes and pores from the lamina.
Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.
A previously unrecognized group of metalloenzymes enables human gut microbes to metabolize dietary molecules and neurotransmitters and likely mediates interactions and metabolism among environmental microorganisms.
Homology information implicit in regions of conserved synteny allows quantification of gene origination by complete sequence divergence, revealing a larger-than-expected role for other mechanisms of origin, including de novo origination.