Genome dilution limits cell growth by modulating the activities, rather than the concentrations, of RNA polymerases and ribosomes, and is accompanied by changes in proteome composition.
Howard Choi, Deborah Simpson ... Chavaunne T Thorpe
Metabolic labelling reveals complex proteome dynamics in tendon, with faster turnover of proteins in the glycoprotein-rich interfascicular matrix compared to the collagen-rich fascicular matrix.
A comprehensive compendium of myelin proteins in the peripheral nervous system has been created, alongside a method to address molecular diversity of myelin sheaths in health and disease.
Jonathan G Van Vranken, Jiaming Li ... Steven P Gygi
Solvent shift assays such as solvent proteome profiling and solvent-PISA are valuable tools for identifying protein-ligand interactions on a proteome-wide scale and can be used to determine drug target engagement, which should benefit future drug discovery efforts.
Han-Hsuan Liu, Daniel B McClatchy ... Hollis T Cline
A screen of visual-experience induced changes in newly-synthesized proteins in Xenopus optic tectum identifies unexpected candidate plasticity proteins.
Maria Pernemalm, AnnSofi Sandberg ... Janne Lehtiö
Comprehensive mass spectrometry analysis of human plasma proteome reveals tissue leakage proteins, describes variability between individual plasma proteomes and demonstrates protein transfer across the placenta during pregnancy.
Biochemical analysis on hetero-mutated c10 subunits ring and molecular dynamics simulations demonstrate the cooperation among c-subunits of FoF1-ATP synthase in rotation-coupled proton translocation.
SIMC1 and SLF1 bind exclusively to SLF2 to form two separate complexes that direct the human SMC5/6 complex to its antiviral defense or DNA lesion repair activities.
The sour receptor, OTOP1, and related proton channel OTOP3 are steeply activated by extracellular protons while OTOP2 is active over a broad pH range and differences in gating are partly attributed to extracellular domains.
