4,945 results found
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    1. Chromosomes and Gene Expression
    2. Microbiology and Infectious Disease

    A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast

    Abbas Jariani et al.
    A new high-throughput method for single-cell RNA-seq in yeast cells shows how stochastic expression of glucose-repressed genes contributes to cell-to-cell differences during adaptation to an environmental change.
    1. Structural Biology and Molecular Biophysics

    Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine

    Wilson Wong et al.
    The molecular mechanism behind how emetine inhibits the ribosome of the human malaria parasite, along with structural details of the complex formed, is revealed at high resolution using cryo-electron microscopy.
    1. Cell Biology
    2. Microbiology and Infectious Disease

    A single-parasite transcriptional atlas of Toxoplasma Gondii reveals novel control of antigen expression

    Yuan Xue et al.
    Single-cell RNA-sequencing resolves the transcriptional landscape of asexual development in Toxoplasma gondii, revealing concerted genetic programs to Plasmodiumfalciparum and a novel transcriptional factor that controls antigen switching.
    1. Structural Biology and Molecular Biophysics

    Routine single particle CryoEM sample and grid characterization by tomography

    Alex J Noble et al.
    Fiducial-less tomography on single particle cryoEM samples reveals that most particles are adsorbed to the air-water interface and allows for researchers to diagnose and solve sample, grid, ice thickness, collection, and processing issues.
    1. Cell Biology

    A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate carrier MPC

    Robinson Arce-Molina et al.
    A user-friendly genetically-encoded fluorescent sensor of improved dynamic range is introduced to facilitate the study of mitochondrial metabolism and diversity.
    1. Neuroscience

    Dreaming with hippocampal damage

    Goffredina Spanò et al.
    A provoked awakening protocol during sleep reveals that selective bilateral hippocampal damage in humans is associated with reduced frequency, quality, and content of dreaming.
    1. Developmental Biology
    2. Neuroscience

    Revisiting the role of Dcc in visual system development with a novel eye clearing method

    Robin J Vigouroux et al.
    A new eye-specific Dcc mutant combined with an improved clearing protocol for the eye and brain (EyeDISCO) reveals the requirement of the receptor Dcc for retinal development and maintenance.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Population genomics reveals the origin and asexual evolution of human infective trypanosomes

    William Weir et al.
    The protozoan parasite Trypanosoma brucei gambiense has undergone recent clonal evolution that reveals the theoretically predicted Meselson effect at a genome-wide level.

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