5,652 results found
    1. Structural Biology and Molecular Biophysics

    Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine

    Wilson Wong et al.
    The molecular mechanism behind how emetine inhibits the ribosome of the human malaria parasite, along with structural details of the complex formed, is revealed at high resolution using cryo-electron microscopy.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Improved CUT&RUN chromatin profiling tools

    Michael P Meers et al.
    Enhancements to the CUT&RUN antibody-targeted nuclease strategy for chromatin profiling include an improved affinity-cleavage fusion protein and digestion protocol, and a simplified calibration strategy.
    1. Immunology and Inflammation

    High-dimensional analysis of intestinal immune cells during helminth infection

    Laura Ferrer-Font et al.
    A systematic optimization of intestinal digestion protocols allows for the isolation and high-dimensional flow cytometric analysis of intestinal immune cells during murine helminth infection.
    1. Chromosomes and Gene Expression
    2. Microbiology and Infectious Disease

    A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast

    Abbas Jariani et al.
    A new high-throughput method for single-cell RNA-seq in yeast cells shows how stochastic expression of glucose-repressed genes contributes to cell-to-cell differences during adaptation to an environmental change.
    1. Structural Biology and Molecular Biophysics

    Routine single particle CryoEM sample and grid characterization by tomography

    Alex J Noble et al.
    Fiducial-less tomography on single particle cryoEM samples reveals that most particles are adsorbed to the air-water interface and allows for researchers to diagnose and solve sample, grid, ice thickness, collection, and processing issues.
    1. Neuroscience

    Dreaming with hippocampal damage

    Goffredina Spanò et al.
    A provoked awakening protocol during sleep reveals that selective bilateral hippocampal damage in humans is associated with reduced frequency, quality, and content of dreaming.
    1. Developmental Biology
    2. Neuroscience

    Revisiting the role of Dcc in visual system development with a novel eye clearing method

    Robin J Vigouroux et al.
    A new eye-specific Dcc mutant combined with an improved clearing protocol for the eye and brain (EyeDISCO) reveals the requirement of the receptor Dcc for retinal development and maintenance.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    A systematically-revised ribosome profiling method for bacteria reveals pauses at single-codon resolution

    Fuad Mohammad et al.
    With the removal of confounding artifacts, ribosome profiling can yield insight into the mechanism of protein synthesis in bacteria at high resolution.
    1. Genetics and Genomics

    Genome-wide quantification of the effects of DNA methylation on human gene regulation

    Amanda J Lea et al.
    mSTARR-seq identifies regions of the genome where DNA methylation causally impacts gene expression, providing a map of which epigenetic marks may influence trait variation.
    1. Developmental Biology
    2. Genetics and Genomics

    Genome editing enables reverse genetics of multicellular development in the choanoflagellate Salpingoeca rosetta

    David S Booth, Nicole King
    Genome editing in the choanoflagellate Salpingoeca rosetta opens newfound possibilities to functionally probe choanoflagellate genes that may illuminate the origin of their closest relatives, the animals.

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