A combination of spatial proteomic and autophagic flux approaches was used to reveal the landscape of turnover of damaged lysosomes, demonstrating a key role for the autophagy receptor TAX1BP1 and its associated kinase TBK1 in both HeLa cells and iNeurons.
Johanna Odenwald, Bernardo Gabiatti ... Susanne Kramer
Proteins that are low abundant and/or poorly accessible to antibodies can be readily localised with fluorescent streptavidin, when expressed fused to a biotin ligase.
Andrea Mair, Shou-Ling Xu ... Dominique C Bergmann
Proximity-labeling using engineered biotin ligases TurboID and miniTurbo enables detection of cell-type-specific and low abundance protein complexes and subcellular proteomes in Arabidopsis and other plants.
BLITZ system enables proximity-dependent biotin labelling in live zebrafish embryos with cell and tissue specificity, providing a versatile and valuable tool for proteomic discovery using the zebrafish model.
Combined cutting-edge technologies discovered a pair of carboxylate transporters that appears evolutionarily different among novel transporters and that is essential for parasite physiology.
APEX2 proximity labeling of genetically targeted neurons enables subcellular and cell type-specific proteomics in the mouse brain, revealing the axonal and somatodendritic proteomes of midbrain dopaminergic neurons.
Super-resolution microscopy, biochemical and functional analyses reveal how α-taxilin and γ-taxilin are assembled at the subdistal appendages and their roles in microtubule organization.
Overexpression of inhibitor of Bruton's tyrosine kinase contributes to the process of tumorigenesis by amplifying translation, represents a promising target for anti-cancer therapies.
