Proximity-labeling using engineered biotin ligases TurboID and miniTurbo enables detection of cell-type-specific and low abundance protein complexes and subcellular proteomes in Arabidopsis and other plants.
The molecular microenvironment of coronaviral replicase complexes provides functional and spatial links between conserved cellular processes and viral RNA synthesis, and highlights potential targets for the development of novel antivirals.
A light-dependent two-hybrid tool with transcriptional readout detects multiple protein-protein interactions in living mammalian cells with high signal-to-background ratios and enables genetic selections.
Integrative structural biology reveals a conformational equilibrium in the Box C/D methylation enzyme that regulates the extent of site-specific 2'-O-rRNA methylation in dependence of the RNA sequence.
Open source software enables neuroscientists to integrate single neuron or synaptic-resolution datasets from different imaging modalities to analyse morphology and connectivity at the scale of whole brains and connectomes.