Proximity-labeling using engineered biotin ligases TurboID and miniTurbo enables detection of cell-type-specific and low abundance protein complexes and subcellular proteomes in Arabidopsis and other plants.
The molecular microenvironment of coronaviral replicase complexes provides functional and spatial links between conserved cellular processes and viral RNA synthesis, and highlights potential targets for the development of novel antivirals.
A light-dependent two-hybrid tool with transcriptional readout detects multiple protein-protein interactions in living mammalian cells with high signal-to-background ratios and enables genetic selections.
The organization of layered/laminated axon projections in specific regions of the fruitfly central brain is regulated by short-range repulsive guidance and is critical for local inhibitory circuit formation and function.
Mutation of Glycine 34 to Arginine within the N-terminal tail of histone H3 alters post-translational modifications on Lysine 36 and is associated with a delay in replication restart, defective homologous recombination and an increase in genomic instability.
A novel microscopy-based assay shows that dendritic cells encountering pathogenic stimuli form increased complexes of specific SNARE proteins, driving release of large amounts of inflammatory cytokines.
The coding sequences of a very highly conserved family of neurogenic transcription factors from different species have evolved to generate proteins that have different life times causing them to display quantitatively different neural induction potentials.