A quantitative live imaging approach unveils that earliest neurogenic progenitors in the vertebrate retina arise from asymmetric divisions and that this asymmetry involves Notch signalling through the endocytic pathway.
A robust fluorescence microscopy-based data acquisition and analysis framework affords the precise measurement of cell surface receptor affinities toward their cognate ligands and their densities in live cells/tissues.
A novel panel of homoFRET biosensors that detect kinase and second messenger activity, called FLAREs, enables multiparameter imaging of signaling activities within a single live cell with a quantitative and ratiometric readout.
Imaging, quantitative immunoblotting and mass spectrometry reveal that hundreds of surface-expressed neuronal membrane proteins exhibit atypical glycosylation profiles, resulting in changes in protein half-life and synaptic responses.
An analysis of review, promotion and tenure documents from 129 US and Canadian universities suggests institutions could better fulfill their public missions by changing how they incentivize the public dimensions of faculty work.