Comprehensive structural and compositional characterization of the in vivo 30S ribosomal assembly landscape reveals parallel pathways for 3′-domain formation and a previously unknown role for the assembly factor RimP.
Confronting different models of chromatin accessibility with temporally resolved transcription profiles favors a scenario where transcription factors actively, rather than passively, drive chromatin from the inaccessible to the accessible state.
A robust fluorescence microscopy-based data acquisition and analysis framework affords the precise measurement of cell surface receptor affinities toward their cognate ligands and their densities in live cells/tissues.
Genome editing, advanced live-cell microscopy, and computational modeling were combined to measure WNT/CTNNB1 signaling parameters at the single molecule level, revealing critical regulatory nodes in this important signal transduction pathway.
A robust method to quantitatively visualize HIV-1 replication complexes in infected cells shows that these complexes remain associated with the viral capsid beyond nuclear import in primary macrophages.
Melanin staining for micro-CT imaging, using a novel application of ionic silver deposition, enables qualitative and quantitative 3D analysis of zebrafish pigmentation and reveals subtle phenotypes missed by light microscopy.
Neocortical synapses in layer 4 of the human temporal lobe neocortex were quantitatively characterized, at the subcellular level, using high-end, high-resolution electron microscopy and 3D-volume reconstructions.
Quantitative 3D lattice light sheet microscopy of unperturbed cells combined with electron tomography and acute loss of function experiments reveals how dynamic ESCRT-III/Vps4 assemblies succeed in reverse membrane budding on endosomes.