477 results found
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components

    Julia P Steringer et al.
    The first reconstitution of an unconventional secretory mechanism uncovered the molecular mechanism by which Fibroblast Growth Factor 2 is secreted from mammalian cells.
    1. Cell Biology

    RNA-directed activation of cytoplasmic dynein-1 in reconstituted transport RNPs

    Mark A McClintock et al.
    Reconstitution of dynein-based mRNA transport with purified components reveals a pivotal role of an RNA localisation signal in controlling the assembly and activity of the translocation machinery.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    The guide sRNA sequence determines the activity level of box C/D RNPs

    Andrea Graziadei et al.
    Integrative structural biology reveals a conformational equilibrium in the Box C/D methylation enzyme that regulates the extent of site-specific 2'-O-rRNA methylation in dependence of the RNA sequence.
    1. Cell Biology
    2. Computational and Systems Biology

    DASC, a sensitive classifier for measuring discrete early stages in clathrin-mediated endocytosis

    Xinxin Wang et al.
    A new user-intervention-free classification, using single-fluorescent markers, measures conventionally unmeasureable phenotypes in early stages during clathrin-mediated endocytosis.
    1. Neuroscience

    Cre-assisted fine-mapping of neural circuits using orthogonal split inteins

    Haojiang Luan et al.
    A genetic method allows neurons to be individually identified and characterized by combining information about both their developmental origins and their mature patterns of gene expression.
    1. Developmental Biology

    Antagonistic regulation by insulin-like peptide and activin ensures the elaboration of appropriate dendritic field sizes of amacrine neurons

    Jiangnan Luo et al.
    Afferent-derived factors control the elaboration of appropriate and robust sizes of dendritic arbors by dynamically modulating dendritic growth parameters.
    1. Cell Biology
    2. Immunology and Inflammation

    Transient protein accumulation at the center of the T cell antigen-presenting cell interface drives efficient IL-2 secretion

    Danielle J Clark et al.
    A membrane-associated, supramolecular protein complex with dynamically changing components, the central supramolecular activation cluster, regulates the generation of the T cell effector cytokine IL-2 depending on its composition.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structural insights into the Ca2+-dependent gating of the human mitochondrial calcium uniporter

    Yan Wang et al.
    Cryo-EM structures of human MCU-EMRE-MICU1-MICU2 complex in the apo and Ca2+-bound states reveal how MICU1 and MICU2 impart their gating function to the mitochondrial calcium uniporter.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    The autophagy adaptor NDP52 and the FIP200 coiled-coil allosterically activate ULK1 complex membrane recruitment

    Xiaoshan Shi et al.
    Hydrogen-deuterium exchange, electron microscopy, and vesicle reconstitution show how binding of the autophagy adaptor NDP52 to the FIP200 subunit of the ULK1 complex triggers membrane binding in autophagy.
    1. Microbiology and Infectious Disease

    Vibrio deploys type 2 secreted lipase to esterify cholesterol with host fatty acids and mediate cell egress

    Suneeta Chimalapati et al.
    Considering the course of a pathogen's evolution, there appears to be interplay between secretion systems, providing unique, synergistic mechanisms to support a successful lifestyle for possibly pathogenesis, symbiosis and/or parasitosis.

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