RIM binding UNC-13L C2A domain releases UNC-13L from an autoinhibitory homodimeric complex to become fusion-competent, and regulates probability of synaptic vesicle release in the post-priming process.
Excitatory synapses that occur further away from the postsynaptic cell soma exhibit greater neurotransmitter release probability, which appears to improve signal transfer fidelity for high-frequency afferent firing.
Synaptophysins and gyrins dampen synaptic strength selectively at low frequencies, hinting that synaptic transmission may play a frequency filtering role in biological computation that is more general than currently envisioned.
Active zone release probability is correlated with calcium channel density and calcium influx at single release sites, with release strength increasing in an activity-dependent manner during synapse maturation.
Release site heterogeneity represents a previously unknown level of structural and functional organization within individual active zones in central synapses, which determines the spatiotemporal dynamics of multi-vesicular release.