Jacob Peter Matson, Raluca Dumitru ... Jeanette Gowen Cook
The unique cell cycle variations of pluripotent stem cells ensures that the first step in DNA replication is particularly fast and this rapid rate, in turn, restrains early differentiation.
hRPA permits the BLM helicase to bidirectionally unwind DNA from a nick which could potentially facilitate its function switching in DNA repair and promote end resection in homologous recombination.
Clifford A Froelich, Sukhyun Kang ... Eric J Enemark
The crystal structure of the MCM helicase bound to single-stranded DNA reveals a binding motif that is critical for cell viability, helicase activation and DNA replication.
Robert LeDesma, Brigitte Heller ... Alexander Ploss
A combined computational, genetic, and biochemical analysis of the hepatitis E virus open reading frame 1 (ORF1) protein identifies a novel domain-domain interaction that is vital for fitness and supports a proposed structural model of the ORF1 polyprotein.
Mark Hedglin, Senthil K Perumal ... Stephen Benkovic
Fluorescence resonance energy transfer has been used to explore the interactions between DNA polymerases, sliding clamps and clamp loaders as DNA is replicated in human cells.
CMG helicase translocates with force while encircling duplex DNA, enabling two opposing CMG complexes at an origin to melt the duplex and switch to encircling separate single strands.
As a cell prepares to divide, a molecular actor known as the Origin Recognition Complex makes intricate ATP-driven movements to recruit proteins required to duplicate DNA.
Systematic analyses of DNA replication machinery components in human cells reveal a requirement of MCM-dependent de novo loading or mobilization of cohesin at replication forks in establishing sister-chromatid cohesion.
