TRAF3, a negative regulator of noncanonical NF-κB signaling, maintains epithelial cell quiescence at confluence, and its loss triggers upregulation of immunity genes and prevents entry into G0 at high cell density.
Plants and humans use a shared mechanism, the eukaryotic metabolic sensor TARGET OF RAPAMYCIN protein kinase and its substrate, an RNA-binding protein called LARP1, to coordinate post-transcriptional gene expression.
The antiviral and genomic DNA deaminase APOBEC3B is repressed in normal cells by PRC1.6/E2F6 and DREAM/E2F4 complexes and deregulation of this axis provides a unifying mechanism for overexpression in cancer.
Regulatory networks of genes controlling different aspects of insect reproduction have been identified by a systems-level analysis of quantitative phenotypic information obtained from the loss of individual cell signaling genes.
In fusion-positive rhabdomyosarcoma, CHD4 positively regulates super-enhancer-mediated gene expression by allowing a chromatin architecture at these cis-regulatory regions, which is permissive to the binding of the transcription factor PAX3-FOXO1.
Short homology arms exposed by CRISPR/Cas9 cleavage can target integration at genomic CRISPR/Cas9 cut sites at high frequencies with reproducible precision using pGTag vectors in zebrafish and mammalian cells.
A combination of in vitro and in vivo experiments demonstrate a cell-autonomous role of the KIT ligand/KIT signaling pathway in protecting retinal photoreceptor cells from environmentally or genetically caused degeneration.