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Biochemical tags can be easily knocked into endogenous genes in mammalian stem cells using optimised and scalable protocols that will enable annotation of protein levels, localisation and interaction partners.

Building on previous work (Jinek et al., 2013), we report a simple and robust system to achieve high fidelity and high efficiency (30% of homologous recombination) genome engineering by homology-directed repair pathway in human cells using cell cycle synchronization and timed delivery of Cas9 ribonucleoprotein complexes.

Transient transcriptome sequencing and kinetic modeling suggest how the kinetics and yield of RNA splicing are encoded in the human genome.

An experimental approach allowing characterization of viral RNA ligands recognized by RIG-I-like receptors during infection by any RNA viruses is established and validated.

The high-resolution structure of a filamentous flexible plant virus shows that there is structural homology between its coat protein and the nucleoproteins of an unrelated group of enveloped RNA animal viruses.

Alternative splicing provides a mechanism to maintain oscillations of the circadian clock.

ChAR-seq is a massively parallelized de novo RNA mapping assay, which is capable of generating hundreds to thousands of RNA-binding maps with no a priori knowledge of target RNAs.

Single molecule experiments reveal competition between spliceosome activation and the discard of the triple small nuclear ribonucleoprotein complex.

The loss of genes that encode RNA splicing factors weakens cancer cells in a way that could be exploited by new approaches to treatment.