A genetic screen reveals that two predicted glycosyltransferases promote rosette development and prevent cell clumping in one of the closest living relatives of animals, the choanoflagellate S. rosetta.
A bioengineering approach identifies tissue morphology as an effective variable for controlling the inception of neural organoid morphogenesis via induction of a biomimetic, singular neural rosette tissue cytoarchitecture.
Genome editing in the choanoflagellate Salpingoeca rosetta opens newfound possibilities to functionally probe choanoflagellate genes that may illuminate the origin of their closest relatives, the animals.
In Drosophila oocytes, the exclusion of the scaffold protein PAR3 from the posterior cortex depends on PAR1 and endocytosis, while its anterior localisation requires microtubules and recycling endosomes.
A combination of high-resolution microscopy and reverse genetics identified key components of the alveolin network playing an essential role in the assembly of subpellicular microtubules and conoid in Toxoplasma gondii..
Extensive benchmarking reveals that errors made when manually building models into near-atomic-resolution cryoEM density may automatically be corrected using an improved Rosetta-based structure refinement method.