Yeast extracts recapitulate a quality-control pathway dedicated to rescuing stalled ribosomes, providing unexpected insights into a noncanonical elongation reaction and the fate of incompletely synthesized proteins.
A SWI/SNF-family chromatin remodeling subcomplex from yeast is identified (RSC1) that is specialized to slide nucleosomes residing on DNA sequences that confer partial nucleosome unwrapping, with assistance from accessory factors.
Analysis of neo-splicetope-specific T cell responses strongly questions the idea that in vitro proteasome-catalyzed peptide splicing reaction simulates the in vivo situation with the same high fidelity as the in vitro generation of non-spliced epitopes.
A hierarchical relationship among highly conserved regulatory ribosomal ubiquitylation events and the presence of antagonistic deubiquitylating enzymes suggests a dynamic ubiquitin code impacts ribosome-associated activities.
Adaptations in protein synthesis and mRNA surveillance machinery enabled the malaria-causing parasite P. falciparum to efficiently and accurately translate long polyA nucleotide runs into long poly-lysine peptides.