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    1. Neuroscience

    Charting brain growth and aging at high spatial precision

    Saige Rutherford, Charlotte Fraza ... Andre F Marquand
    During an individual's lifetime, their brain undergoes a unique transformation that we have precisely mapped across thousands of people to create and share reference growth charts of the human brain's lifespan which allow for single subject exploration and inference.
    1. Biochemistry and Chemical Biology

    High-throughput proteomics of nanogram-scale samples with Zeno SWATH MS

    Ziyue Wang, Michael Mülleder ... Markus Ralser
    Zeno SWATH MS, a novel data-independent acquisition technique in proteomics, which by facilitating sensitive detection in high-throughput experiments with low sample amounts, mitigates current bottlenecks of high-throughput proteomics.
    1. Medicine

    Meta-research: justifying career disruption in funding applications, a survey of Australian researchers

    Adrian Barnett, Katie Page ... Susanna Cramb
    A survey of health and medical researchers revealed important diffrences in how career disruption is treated in funding decisions, with differences in what applicants with disruption are willing to divulge and the adjustments made by reviewers.
    1. Chromosomes and Gene Expression

    Efficient chromatin accessibility mapping in situ by nucleosome-tethered tagmentation

    Steven Henikoff, Jorja G Henikoff ... Kami Ahmad
    A simple modification of CUT&Tag chromatin profiling method provides high-quality chromatin accessibility maps and indicates that 'open' chromatin results from a transcription-coupled process at promoters and enhancers.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Microstructural differences in the osteochondral unit of terrestrial and aquatic mammals

    Irina AD Mancini, Riccardo Levato ... Jos Malda
    Analysis of the osteochondral unit of aquatic mammals reveals a distinctly different tissue structure, lacking the arcade-like collagen fiber organization, a calcified cartilage layer and a dense subchondral bone plate, typically seen in osteochondral tissue of terrestrial mammals.
    1. Cell Biology
    2. Developmental Biology

    Science Forum: Imaging methods are vastly underreported in biomedical research

    Guillermo Marqués, Thomas Pengo, Mark A Sanders
    The poor reporting of imaging methods in the scientific literature is hindering the evaluation and replication of biomedical research.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Demographic reconstruction from ancient DNA supports rapid extinction of the great auk

    Jessica E Thomas, Gary R Carvalho ... Michael Knapp
    Reconstruction of great auk population dynamics suggests that hunting pressure alone could have been responsible for their extinction, demonstrating that even abundant, widespread species can be vulnerable to intense exploitation.
  1. Research Culture: A survey of early-career researchers in Australia

    Katherine Christian, Carolyn Johnstone ... Michael R Doran
    Job insecurity is putting stress on early-career researchers in Australia, compromising their career development and potentially reducing the quality of research.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues

    Tin Ki Tsang, Eric A Bushong ... Mark H Ellisman
    A broadly applicable method that faithfully preserves genetically labeled cellular structures for 3D electron microscopy (EM) and correlated light and electron microscopy (CLEM).
    1. Structural Biology and Molecular Biophysics

    Routine single particle CryoEM sample and grid characterization by tomography

    Alex J Noble, Venkata P Dandey ... Bridget Carragher
    Fiducial-less tomography on single particle cryoEM samples reveals that most particles are adsorbed to the air-water interface and allows for researchers to diagnose and solve sample, grid, ice thickness, collection, and processing issues.