New open-source method contains all the tools for extracting open chromatin or RNA from thousands of single cells in parallel for <5% of the cost of state-of-the-art commercial alternatives, paving the way for whole-tissue atlasing and new protocol development.

Single-cell chromatin accessibility analysis reveals the intricate regulatory landscape of mouse testicular development, uncovering novel cell subpopulations and transcription factors, and offering valuable insights into the molecular mechanisms driving germ cell and somatic cell maturation.

The combination of single-cell analysis and cell fate mapping studies evidenced the presence of AIRE-expressing transit-amplifying thymic epithelial cells, which differentiate into mature medullary thymic epithelial cells expressing tissue-specific antigens.

Simultaneous multimodal measurement of chromatin accessibility, gene expression, and protein expression provides a unified view of the molecular underpinnings of immune cell state in health and disease.

Chromatin-based cancer stem cell heterogeneity typifies primary glioblastoma and includes an invasive subtype associated with a lower survival.

Epigenome and Mitochondrial Barcode of Lineage from Endogenous Mutations (EMBLEM) enable tracking cell lineage in combination with chromatin profile in ATAC-seq data.

In vivo modulation of the prostaglandin, interferon, or granulocyte colony-stimulating factor pathway induces rapid and specific transcriptional changes in hematopoietic stem cells with significant heterogeneity in the cellular response.

A novel population of adipocytes that play essential roles in adaptive thermogenesis via cell autonomous and non-autonomous effects and regulate systemic glucose metabolism in mice.

A single-cell RNA profiling analysis has delineated the molecular changes driving the progression from actinic keratosis to cutaneous squamous cell carcinoma.