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    1. Cancer Biology
    2. Cell Biology

    Intravital deep-tumor single-beam 3-photon, 4-photon, and harmonic microscopy

    Gert-Jan Bakker, Sarah Weischer ... Peter Friedl
    Infrared high-pulse-energy low-pulse-repetition-rate excitation advances deep intravital microscopy in strongly scattering tissues such as skin tumors and thick bone, thereby bringing previously inaccessible tumor areas in reach with subcellular resolution.
    1. Cell Biology

    A tRNA processing enzyme is a key regulator of the mitochondrial unfolded protein response

    James P Held, Gaomin Feng ... Maulik R Patel
    A key role for RNAs in sensing and mounting a response to mitochondrial stress.
    1. Cell Biology

    Simultaneous recording of multiple cellular signaling events by frequency- and spectrally-tuned multiplexing of fluorescent probes

    Michelina Kierzek, Parker E Deal ... Christoph Brenker
    FASTM is a novel technique that leverages phase-sensitive signal detection for time-resolved multiplexing of probes with millisecond time resolution, opening new opportunities for interrogating rapid cellular signaling and (bio)chemical reactions.
    1. Stem Cells and Regenerative Medicine

    Kidney organoids recapitulate human basement membrane assembly in health and disease

    Mychel RPT Morais, Pinyuan Tian ... Rachel Lennon
    Kidney organoids are a high-fidelity system for investigating basement membrane regulation in development and disease.
    1. Cell Biology

    Super-resolution microscopy reveals majorly mono- and dimeric presenilin1/γ-secretase at the cell surface

    Abril Angélica Escamilla-Ayala, Ragna Sannerud ... Wim Annaert
    Super-resolution microscopy sets a new strategy to comprehend the membrane organization of γ-secretase at single complex resolution identifying nanodomain associations and its diffusion in situ in the living membrane.
    1. Cell Biology

    Enhanced single RNA imaging reveals dynamic gene expression in live animals

    Yucen Hu, Jingxiu Xu ... Weirui Ma
    The combination of the MS2 system with the Suntag system as a signal amplifier allows enhanced single-molecule mRNA imaging in live cells and animals.
    1. Cell Biology
    2. Developmental Biology

    The centrosomal protein 83 (CEP83) regulates human pluripotent stem cell differentiation toward the kidney lineage

    Fatma Mansour, Christian Hinze ... Kai M Schmidt-Ott
    CEP83 plays a central role in regulating the development of intermediate mesoderm (IM) nephron progenitors, which may involve direct effects of CEP83 in the nephron progenitor differentiation program and indirect lateral plate mesoderm-mediated effects on the IM.
    1. Developmental Biology
    2. Neuroscience

    Cd59 and inflammation regulate Schwann cell development

    Ashtyn T Wiltbank, Emma R Steinson ... Sarah Kucenas
    Cd59 and developmental inflammation are key players in myelinating glial cell development, highlighting the collaboration between glia and the innate immune system to ensure normal neural development.
    1. Ecology

    Algal-fungal symbiosis leads to photosynthetic mycelium

    Zhi-Yan Du, Krzysztof Zienkiewicz ... Gregory M Bonito
    The capacity for symbiosis between photosynthetic microalgae and early diverging lineages fungi was demonstrated with microscopy and stable isotope exchange of carbon and nitrogen.
    1. Cell Biology
    2. Plant Biology

    Developmental patterning function of GNOM ARF-GEF mediated from the cell periphery

    Maciek Adamowski, Ivana Matijević, Jiří Friml
    The plant developmental regulator GNOM performs its unique function from the cell periphery, through sequence features present in all its domains, and not by the control of endocytosis.