5,380 results found
    1. Structural Biology and Molecular Biophysics
    2. Neuroscience

    Single-molecule fluorimetry and gating currents inspire an improved optical voltage indicator

    Jeremy S Treger et al.
    ArcLight, a popular optogenetic reporter of voltage, is studied at both single-molecule and macroscopic levels, which leads to new mechanistic understanding and to the rational design of a faster reporter.
    1. Structural Biology and Molecular Biophysics
    2. Chromosomes and Gene Expression

    Single-molecule tracking of the transcription cycle by sub-second RNA detection

    Zhengjian Zhang et al.
    A new technique called fastFISH enables nearly real-time and stoichiometric detection of nascent RNA and the tracking of individual stages of transcription at the level of single-molecules.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Differences in interactions between transmembrane domains tune the activation of metabotropic glutamate receptors

    Jordana K Thibado et al.
    Homo- and heterodimeric group II metabotropic glutamate receptors show diverse modes of interaction between transmembrane domains that control their distinct assembly and activation properties.
    1. Structural Biology and Molecular Biophysics
    2. Neuroscience

    Complexin induces a conformational change at the membrane-proximal C-terminal end of the SNARE complex

    Ucheor B Choi et al.
    Complexin can have two conformations when bound to a ternary SNARE complex, one of which induces a conformational change of the SNARE complex at the C-terminus.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Cellular encoding of Cy dyes for single-molecule imaging

    Lilia Leisle et al.
    Cyanine fluorophores are encoded as non-canonical amino acids to produce functional proteins in cell-free translation systems and live cells for single-molecule imaging.
    1. Structural Biology and Molecular Biophysics

    Structural dynamics of E. coli single-stranded DNA binding protein reveal DNA wrapping and unwrapping pathways

    Sukrit Suksombat et al.
    Single-molecule force and fluorescence spectroscopy reveal the structural states and dynamics of E. coli single-stranded DNA binding proteins and the energy landscape of the nucleo–protein complex.
    1. Computational and Systems Biology
    2. Structural Biology and Molecular Biophysics

    Conformational dynamics in TRPV1 channels reported by an encoded coumarin amino acid

    Ximena Steinberg et al.
    Single-molecule measurement of conformational dynamics using a genetically encoded fluorescent probe suggests that the selectivity filter region of TRPV1 channels undergoes dynamic motion during agonist activation.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Real-time observation of signal recognition particle binding to actively translating ribosomes

    Thomas R Noriega et al.
    The signal recognition particle only stably engages translating ribosome nascent-chain complexes exposing a functional signal sequence.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Dynamics and consequences of spliceosome E complex formation

    Joshua Donald Larson, Aaron A Hoskins
    Single molecule fluorescence analysis of spliceosome E complex formation reveals how tuning of U1 binding to the 5' SS facilitates efficient capture of the snRNP by pre-mRNAs.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Labeling proteins inside living cells using external fluorophores for microscopy

    Kai Wen Teng et al.
    A toxin is used to introduce an otherwise cell-impermeant fluorophore-antibody (or some thing which is equally specific) to bind to an intracellular protein which allows for super resolution imaging and single particle tracking inside the living cell.

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