5,294 results found
    1. Physics of Living Systems

    Single molecule microscopy reveals key physical features of repair foci in living cells

    Judith Miné-Hattab et al.
    Upon DNA damage, Rad52 forms a membraneless sub-compartment where Rad52 molecules are highly dynamic and share properties with liquid-liquid phase-separated molecules, reflecting the existence of a liquid droplet around damaged DNA.
    1. Cell Biology
    2. Physics of Living Systems

    The transition state and regulation of γ-TuRC-mediated microtubule nucleation revealed by single molecule microscopy

    Akanksha Thawani et al.
    Four αβ-tubulin dimers associate laterally on γ-TuRC to define the rate-limiting transition state for microtubule nucleation.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Cellular encoding of Cy dyes for single-molecule imaging

    Lilia Leisle et al.
    Cyanine fluorophores are encoded as non-canonical amino acids to produce functional proteins in cell-free translation systems and live cells for single-molecule imaging.
    1. Structural Biology and Molecular Biophysics

    Kinetics of HIV-1 capsid uncoating revealed by single-molecule analysis

    Chantal L Márquez et al.
    Disassembly of the HIV-1 capsid is a catastrophic process, whereby initiation and propagation can be controlled independently by molecules that bind to different features of the capsid lattice.
    1. Biochemistry and Chemical Biology

    Imaging and energetics of single SSB-ssDNA molecules reveal intramolecular condensation and insight into RecOR function

    Jason C Bell et al.
    Individual SSB-ssDNA complexes undergo reversible condensation and de-condensation that is modulated by RecOR during recombination.
    1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    ParB dynamics and the critical role of the CTD in DNA condensation unveiled by combined force-fluorescence measurements

    Julene Madariaga-Marcos et al.
    A single-molecule biophysical approach reveals that the C-terminal domain of ParB blocks ParB network formation by heterodimerization with the full-length protein, which remains bound to the DNA.
    1. Structural Biology and Molecular Biophysics

    2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy

    Melody G Campbell et al.
    Water molecules and amino-acid side chain rotamers have been resolved in the cryoEM structure of T20S proteasome at 2.8 angstroms.
    1. Chromosomes and Gene Expression

    Transcription factor clusters regulate genes in eukaryotic cells

    Adam JM Wollman et al.
    Transcription factors form clusters independently of the presence of DNA, which regulate target genes as opposed to individual monomers, addressing a longstanding question of how transcription factors can find gene targets so quickly.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Molecular basis of outer kinetochore assembly on CENP-T

    Pim J Huis in 't Veld et al.
    The centromeric protein CENP-T assembles the microtubule-binding interface of kinetochores through direct recruitment of two Ndc80 complexes and indirect recruitment of a third one through the Mis12 complex.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Functional instability allows access to DNA in longer transcription Activator-Like effector (TALE) arrays

    Kathryn Geiger-Schuller et al.
    Single molecule DNA-binding trajectories and deterministic modeling analyses demonstrate a functional role for high energy partly folded states in Transcription Activator-Like Effectors that could improve future TALEN design.

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