Fiducial-less tomography on single particle cryoEM samples reveals that most particles are adsorbed to the air-water interface and allows for researchers to diagnose and solve sample, grid, ice thickness, collection, and processing issues.

Spot-On is an easy-to-use website that makes a rigorous and bias-corrected modeling framework for analysis of single-molecule tracking experiments available to all.

A freely available software environment on Amazon's cloud computing network makes processing of cryo-EM data accessible and affordable.

Tracking proteins in live cells is challenging due to technical limitations and biological complexity, but approaches based in Bayesian nonparametrics stand a decent chance at recovering a target protein's dynamic profile from noisy data.

The nanoscopic landscape of the membrane has a direct impact on the spatiotemporal, and likely also the signaling, dynamics of Ras proteins.

The resolution of electron cryo-microscopy can be improved by using optimal exposure values to filter video frames.

cisTEM enables fast processing of single-particle cryo-EM data on CPU-based workstations and is easily accessible to new users.

Multi-body refinement in RELION allows high-resolution reconstruction and visualisation of molecular motions in cryo-EM single-particle data sets with continuous forms of structural heterogeneity.

New functionality in RELION-4.0 allows convenient structure determination from cryo electron tomography data to resolutions sufficient for de novo atomic modelling.

The Volta phase plate enables in-focus single particle analysis at near atomic resolutions, which could expand the capabilities of cryo-EM, especially for small, flexible or heterogeneous macromolecular samples.