Single synaptic vesicle imaging shows that kinetically distinct endocytic pathways are differentially regulated by calcium and temperature, and influence the fidelity of synaptic vesicle protein retrieval.
Real-time monitoring of oxytocin-loaded vesicles and synaptic actin dynamics in zebrafish reveal that Slit3-Robo2-Cdc42 signalling maintains steady-state levels of mature oxytocin neuropeptide readily primed to be secreted upon physiological demand.
Release site heterogeneity represents a previously unknown level of structural and functional organization within individual active zones in central synapses, which determines the spatiotemporal dynamics of multi-vesicular release.
Genetic and electrophysiological analyses reveal that the mechanisms orchestrating the induction and expression of homeostatic plasticity are compartmentalized and operate with exquisite specificity on both sides of the synapse.
The mobilization or silencing of two heterogeneous pools of synaptic vesicles via different frequencies probably enables granule cell to Purkinje cell synapses to better discriminate between the high-rate code of sensory information and background noise.