5,206 results found
    1. Structural Biology and Molecular Biophysics
    2. Chromosomes and Gene Expression

    Single-molecule tracking of the transcription cycle by sub-second RNA detection

    Zhengjian Zhang et al.
    A new technique called fastFISH enables nearly real-time and stoichiometric detection of nascent RNA and the tracking of individual stages of transcription at the level of single-molecules.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Cellular encoding of Cy dyes for single-molecule imaging

    Lilia Leisle et al.
    Cyanine fluorophores are encoded as non-canonical amino acids to produce functional proteins in cell-free translation systems and live cells for single-molecule imaging.
    1. Computational and Systems Biology
    2. Structural Biology and Molecular Biophysics

    Linking time-series of single-molecule experiments with molecular dynamics simulations by machine learning

    Yasuhiro Matsunaga, Yuji Sugita
    A general machine learning scheme for integrating time-series data from single-molecule experiments and molecular dynamics simulations is proposed and successfully demonstrated for the folding dynamics of the WW domain.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Resolving dual binding conformations of cellulosome cohesin-dockerin complexes using single-molecule force spectroscopy

    Markus A Jobst et al.
    A single-molecule approach is able to discriminate the redundant binding conformations of cellulosomal dockerin based on differences in their mechanical properties.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Single-molecule studies contrast ordered DNA replication with stochastic translesion synthesis

    Gengjing Zhao et al.
    Competing DNA polymerases at the DNA sliding clamp are revealed by single-molecule co-localization studies.
    1. Structural Biology and Molecular Biophysics

    Direct single molecule measurement of TCR triggering by agonist pMHC in living primary T cells

    Geoff P O'Donoghue et al.
    Quantitative observations of single-molecule binding between antigen and T cell receptor (TCR) in living primary T cells reveals unbinding kinetics, stoichiometry and signaling molecule recruitment, providing insights into the mechanisms of antigen recognition by the immune system.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Single molecule analysis reveals reversible and irreversible steps during spliceosome activation

    Aaron A Hoskins et al.
    Single molecule experiments reveal competition between spliceosome activation and the discard of the triple small nuclear ribonucleoprotein complex.
    1. Structural Biology and Molecular Biophysics
    2. Chromosomes and Gene Expression

    Live-cell single-molecule tracking reveals co-recognition of H3K27me3 and DNA targets polycomb Cbx7-PRC1 to chromatin

    Chao Yu Zhen et al.
    Live-cell single-molecule tracking reveals that hierarchical cooperation within the Polycomb Cbx7 protein between the low-affinity H3K27me3-binding module and the high-affinity DNA-binding cassette targets Polycomb repressive complex 1 to chromatin.
    1. Structural Biology and Molecular Biophysics
    2. Neuroscience

    Single-molecule fluorimetry and gating currents inspire an improved optical voltage indicator

    Jeremy S Treger et al.
    ArcLight, a popular optogenetic reporter of voltage, is studied at both single-molecule and macroscopic levels, which leads to new mechanistic understanding and to the rational design of a faster reporter.

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