An open-source user-friendly toolbox implementing machine learning for single-molecule FRET analysis enabling experts and non-experts to reproducibly provide dynamic structural biology insights.
A new analysis algorithm (DISC) enables accurate analysis of data from high-throughput single-molecule paradigms and reveals a non-cooperative binding mechanism of cyclic nucleotide-binding domains from HCN ion channels.
A combination of molecular dynamics simulations and X-ray diffraction data has been used to construct more realistic models of proteins and to provide new insights into their interactions with other proteins and biomolecules.
Dorsal raphe Pet1 neurons are molecularly heterogeneous, comprising as many as fourteen distinct subtypes that show biased cell body distributions across dorsal raphe subdomains.
A novel single-molecule sequencing method reveals previously unappreciated heterogeneity in nucleosome positioning on individual chromatin fibers across the human genome.
The rate of DNA unwinding by RecQ helicases is dramatically modulated by the DNA duplex stability in a geometry-dependent manner, providing an intrinsic mechanism for suppressing illegitimate recombination.
Multi-body refinement in RELION allows high-resolution reconstruction and visualisation of molecular motions in cryo-EM single-particle data sets with continuous forms of structural heterogeneity.
The animal phylogeny of glutamate receptors indicates that vertebrate types do not account for all receptor classes originated during evolution, neither are they the pinnacle of a linear evolutive process.
