Cyanine fluorophores are encoded as non-canonical amino acids to produce functional proteins in cell-free translation systems and live cells for single-molecule imaging.
Lattice light-sheet single-molecule imaging shows 3D Sox2 enhancer clusters in live embryonic stem cells and reveals a model linking 3D spatial distribution of cis-elements, differential target search features and localized gene regulation.
New labeling approaches for single-molecule fluorescence microscopy detect the monomeric catalytic subunit of human telomerase within cellular holoenzyme and minimal active recombinant enzyme.
New insights into the mechanism responsible for the nucleation of branches on networks of actin filaments shed light on a process that is essential for eukaryotic cell motility and endocytosis.
In vivo single-molecule imaging demonstrates that Smc5/6 chromatin association depends on ATP hydrolysis and dsDNA binding, requires Nse6 and is modulated by Brc1 after DNA damage.
Single molecular imaging of MeCP2 at high spatial/temporal resolution identifies distinct structural domains contributing to its dynamic behaviors and chromatin interactions in live neurons.
Disassembly of the HIV-1 capsid is a catastrophic process, whereby initiation and propagation can be controlled independently by molecules that bind to different features of the capsid lattice.
The inositol phosphate IP6 is selectively packaged into HIV virions, where it coordinates electropositive pores in the capsid to prevent spontaneous collapse and promote encapsidated DNA synthesis.
The probability of transcription factors binding to their target sites is choreographed through the formation of dynamic multi-protein hubs that transiently interact with actively transcribing genes.
