4,363 results found
    1. Structural Biology and Molecular Biophysics

    Cryo-EM single particle analysis with the Volta phase plate

    Radostin Danev, Wolfgang Baumeister
    The Volta phase plate enables in-focus single particle analysis at near atomic resolutions, which could expand the capabilities of cryo-EM, especially for small, flexible or heterogeneous macromolecular samples.
    1. Structural Biology and Molecular Biophysics

    Using the Volta phase plate with defocus for cryo-EM single particle analysis

    Radostin Danev et al.
    Building on previous work in cryo-electron microscopy (Entchev et al, 2015), it shown that a combination of the Volta phase plate and a small amount of defocus can simplify the experimental set-up, increase the data acquisition rate and improve resolution.
    1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    Robust model-based analysis of single-particle tracking experiments with Spot-On

    Anders S Hansen et al.
    Spot-On is an easy-to-use website that makes a rigorous and bias-corrected modeling framework for analysis of single-molecule tracking experiments available to all.
    1. Structural Biology and Molecular Biophysics

    Mechanisms of ribosome stalling by SecM at multiple elongation steps

    Jun Zhang et al.
    Nascent regulatory peptides tune the translation rate through a continuous, dynamic reshaping of the functional center of the ribosome.
    1. Structural Biology and Molecular Biophysics

    Beam-induced motion correction for sub-megadalton cryo-EM particles

    Sjors HW Scheres
    Building on previous work (Bai et al., 2013), we describe an algorithm that allows cryo-EM structure determination to near-atomic resolution for protein complexes as small as 170 kDa.
    1. Structural Biology and Molecular Biophysics

    Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud

    Michael A Cianfrocco, Andres E Leschziner
    A freely available software environment on Amazon's cloud computing network makes processing of cryo-EM data accessible and affordable.
    1. Structural Biology and Molecular Biophysics

    Cryo-EM: Protein complexes in focus

    Robert M Glaeser
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    1. Structural Biology and Molecular Biophysics

    Microscopy: Cryo-EM enters a new era

    Werner Kühlbrandt
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