Polyunsaturated fatty acids enhance desensitization in pentameric ligand-gated ion channels.

Pulse electron paramagnetic resonance on intact Escherichia coli reveals a substrate-induced structural transition in BtuB, the vitamin B₁₂ transporter, which is not seen in reconstituted membranes and indicates a mechanism of transport.

LptC regulates the LPS entry gate in LptB₂FG, likely through a dynamic behavior of its transmembrane helix, while the β-jellyroll domains are tethered in the periplasm.

Snf7 crystal structures reveal the mechanisms of ESCRT-III activation and polymerization into membrane-remodeling filaments.

Multimodal spectroscopy (smFRET, EPR, SAXS, and SANS) and integrative structural modeling reveal large-scale domain rearrangements in human guanylate binding protein 1 (hGBP1) that are driving forces for the formation of oligomers that enable its biological function in innate immune defense.

A facile method is implemented to modify membrane proteins with biophysical reporters using cysteine-independent approaches in live cells.

Proximity-labeling using engineered biotin ligases TurboID and miniTurbo enables detection of cell-type-specific and low abundance protein complexes and subcellular proteomes in Arabidopsis and other plants.

Methodology for the in vitro assembly of multimeric membrane proteins and the utility of the approach for generating heteromeric variants of homo-multimeric proteins is described.

Methods that use puromycin to localize active translation do not actually detect the site of translation.

Complementing experimental data from EPR spectroscopy with computational modeling techniques provides access to protein structural dynamics and enables the characterization of rare protein conformations.