933 results found
    1. Structural Biology and Molecular Biophysics

    DeepFRET, a software for rapid and automated single-molecule FRET data classification using deep learning

    Johannes Thomsen et al.
    An open-source user-friendly toolbox implementing machine learning for single-molecule FRET analysis enabling experts and non-experts to reproducibly provide dynamic structural biology insights.
    1. Plant Biology

    FRET kinase sensor development reveals SnRK2/OST1 activation by ABA but not by MeJA and high CO2 during stomatal closure

    Li Zhang et al.
    Development of a real-time SnRK2 kinase FRET reporter reveals rapid SnRK2 activation by ABA, but not by Methyl-Jasmonate or elevated CO2, while directly demonstrating basal SnRK2 activity in guard cells.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    In vitro FRET analysis of IRE1 and BiP association and dissociation upon endoplasmic reticulum stress

    Megan C Kopp et al.
    Quantitative FRET UPR induction assay is used to measure IRE1 and BIP association and dissociation by a variety of ER misfolded proteins and by an important BiP substrate-binding domain mutant, significantly enhancing the evidence for the allosteric UPR induction model.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    A FRET sensor of C-terminal movement reveals VRAC activation by plasma membrane DAG signaling rather than ionic strength

    Benjamin König et al.
    VRAC activation, observed with a FRET sensor of intracellular LRRC8-domains movement during gating and by fluorometry, requires plasma membrane localization and diacylglycerol signaling, but is independent of intracellular ionic strength.
    1. Structural Biology and Molecular Biophysics

    Single-molecule view of coordination in a multi-functional DNA polymerase

    Raymond F Pauszek III et al.
    The primer extension and 5' flap processing activities of DNA polymerase I are physically coordinated by combined movements of the DNA substrate and the 5' nuclease domain of the enzyme.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

    Eitan Lerner et al.
    A summary of the current “state-of-the-field” of single-molecule FRET used for probing biomolecular structural dynamics.
    1. Cell Biology
    2. Plant Biology

    FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis

    Rainer Waadt et al.
    Fluorescent sensors can track the movement and distribution of the plant hormone abscisic acid in roots and leaves in Arabidopsis.
    1. Structural Biology and Molecular Biophysics

    Observation of long-range tertiary interactions during ligand binding by the TPP riboswitch aptamer

    Van K Duesterberg et al.
    The combined use of optical trapping and single-molecule FRET permits the study of riboswitch structure formation and conformational dynamics at the same time.
    1. Biochemistry and Chemical Biology

    Stepwise nucleosome translocation by RSC remodeling complexes

    Bryan T Harada et al.
    Single molecule FRET reveals that the SWI/SNF-family remodeling enzyme RSC translocates nucleosomes along the DNA in steps of ~1-2 base pairs in size.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers

    Marijn de Boer et al.
    Selective import via bacterial ABC importers is facilitated by a hitherto unrecognized complexity in the conformational dynamics of the substrate-binding proteins.

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