NMR-based flux measurements show that both bacterial and human Hsp70 chaperones interact with helical, as well as sheet substrates predominantly through a conformational selection mechanism.
Lise M Sjøgaard-Frich, Andreas Prestel ... Stine Falsig Pedersen
NHE1-CaM complexes of multiple stoichiometries regulate cellular Ca2+-dependent NHE1 activity and can contribute to NHE1 dimerization, the latter shown by the NMR structure of CaM linking two NHE1 cytosolic tails.
Biochemical and structural biological analyses show how bacteria can recognize hormones that control the production of important secondary metabolites such as antiparasitics and antibiotics.
The differential conformational space accessible to the two domains of TRBP, even though they both adopt a canonical dsRBD fold, affect how they interact with target RNAs.
Shun Kishimoto, Jeffrey R Brender ... Murali C Krishna
Using noise suppression, real-time imaging of glucose metabolism is possible by 13C MRI and reveals previously undetected metabolic differences within tumors.
Theodoros K Karamanos, Matthew P Jackson ... Sheena E Radford
Solution NMR provides structural and kinetic information about oligomers on pathway to amyloid fibrils that are precisely structured but not cytotoxic.
Nuclear magnetic resonance analysis revealed the structural and dynamical changes in MgtE during the channel closure caused by the cooperative Mg2+-binding, which had remained undescribed only by a static crystal structure.
Chandra M Khantwal, Sherwin J Abraham ... Merritt Maduke
The characterization of a previously unidentified “outward-facing open” conformational state provides a new framework for understanding the CLC transport mechanism.