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    1. Structural Biology and Molecular Biophysics

    Conserved conformational selection mechanism of Hsp70 chaperone-substrate interactions

    Ashok Sekhar, Algirdas Velyvis ... Lewis E Kay
    NMR-based flux measurements show that both bacterial and human Hsp70 chaperones interact with helical, as well as sheet substrates predominantly through a conformational selection mechanism.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Dynamic Na+/H+ exchanger 1 (NHE1) – calmodulin complexes of varying stoichiometry and structure regulate Ca2+-dependent NHE1 activation

    Lise M Sjøgaard-Frich, Andreas Prestel ... Stine Falsig Pedersen
    NHE1-CaM complexes of multiple stoichiometries regulate cellular Ca2+-dependent NHE1 activity and can contribute to NHE1 dimerization, the latter shown by the NMR structure of CaM linking two NHE1 cytosolic tails.
    1. Evolutionary Biology
    2. Structural Biology and Molecular Biophysics

    Indirect sexual selection drives rapid sperm protein evolution in abalone

    Damien Beau Wilburn, Lisa M Tuttle ... Willie J Swanson
    An NMR-based structural study of sperm proteins reveals a novel protein packaging/dispersion system embedded in a coevolutionary arms race.
    1. Structural Biology and Molecular Biophysics

    Enhanced specificity mutations perturb allosteric signaling in CRISPR-Cas9

    Lukasz Nierzwicki, Kyle W East ... Giulia Palermo
    Allostery holds critical role in CRISPR-Cas9 specificity enhancement.
    1. Biochemistry and Chemical Biology

    Biochemical basis for the regulation of biosynthesis of antiparasitics by bacterial hormones

    Iti Kapoor, Philip Olivares, Satish K Nair
    Biochemical and structural biological analyses show how bacteria can recognize hormones that control the production of important secondary metabolites such as antiparasitics and antibiotics.
    1. Structural Biology and Molecular Biophysics

    Differential conformational dynamics in two type-A RNA-binding domains drive the double-stranded RNA recognition and binding

    Firdousi Parvez, Devika Sangpal ... Jeetender Chugh
    The differential conformational space accessible to the two domains of TRBP, even though they both adopt a canonical dsRBD fold, affect how they interact with target RNAs.
    1. Cancer Biology

    Imaging of glucose metabolism by 13C-MRI distinguishes pancreatic cancer subtypes in mice

    Shun Kishimoto, Jeffrey R Brender ... Murali C Krishna
    Using noise suppression, real-time imaging of glucose metabolism is possible by 13C MRI and reveals previously undetected metabolic differences within tumors.
    1. Structural Biology and Molecular Biophysics

    Structural mapping of oligomeric intermediates in an amyloid assembly pathway

    Theodoros K Karamanos, Matthew P Jackson ... Sheena E Radford
    Solution NMR provides structural and kinetic information about oligomers on pathway to amyloid fibrils that are precisely structured but not cytotoxic.
    1. Structural Biology and Molecular Biophysics

    Functional roles of Mg2+ binding sites in ion-dependent gating of a Mg2+ channel, MgtE, revealed by solution NMR

    Tatsuro Maruyama, Shunsuke Imai ... Masanori Osawa
    Nuclear magnetic resonance analysis revealed the structural and dynamical changes in MgtE during the channel closure caused by the cooperative Mg2+-binding, which had remained undescribed only by a static crystal structure.
    1. Structural Biology and Molecular Biophysics

    Revealing an outward-facing open conformational state in a CLC Cl/H+ exchange transporter

    Chandra M Khantwal, Sherwin J Abraham ... Merritt Maduke
    The characterization of a previously unidentified “outward-facing open” conformational state provides a new framework for understanding the CLC transport mechanism.