Biochemical, single molecule, cell and structural biology studies reveal an interaction between the kinesin-5 tail and motor domains regulating high-force production, which is critical for microtubule sliding motility.

PTBP2 ensures that adult protein variants are expressed only in mature neurons through regulation of alternative splicing during early neuronal development.

The structural model of hnRNP A1 shows that it can bind with both RRMs to RNA, which is shown to be relevant for the SMN2 exon 7 splicing mechanism in vivo.

The unfolded protein response sensor/transducer IRE1-mediated splicing of XBP1 mRNA encoding its active downstream transcription factor to maintain the homeostasis of the endoplasmic reticulum is sufficient for growth and development of medaka fish.

Hinokiflavone is identified as a splicing modulator that blocks progression from spliceosome complex A to complex B and inhibits SUMO protease SENP1, causing hyper-SUMOylation affecting 6 U2 snRNP proteins.

Alternative splicing of the Dcc gene, which is controlled by the NOVA RNA-binding proteins, is crucial during neuronal migration and axon guidance.

Extensive periodic regulation of alternative splicing during the cell cycle in genes is linked to cell cycle functions, and involves an auto-inhibitory mechanism that uses the protein kinase CLK1.

Structural and functional analyses show how the spliceosomal Prp3 protein concomitantly binds double- and single- stranded regions in U4/U6 di-snRNAs and serves to stabilize the U4/U6•U5 tri-snRNP for splicing.

A single human cell makes 10-100 transcriptional errors per second; these errors affect splicing and may influence the evolution of coding sequences.

Endothelial cells express a soluble isoform of the L1CAM cell adhesion molecule that is generated by the splicing factor NOVA2 and induces angiogenesis, with relevant implications for ovarian cancer vascularization.