A split-GAL4 collection provides precise genetic targeting of 138 neuronal cell types in the subesophageal zone of adult Drosophila melanogaster.

The generation and systematic characterisation of driver lines labelling a large number of neurons in the Drosophila innate olfactory processing centre bridges electron microscopy neuronal reconstructions, circuits and behaviour.

Systematic analysis of descending neuron anatomy reveals the basic functional map of descending sensory-motor pathways in flies and provides genetic tools for targeted interrogation of neural circuits.

A genetic method allows neurons to be individually identified and characterized by combining information about both their developmental origins and their mature patterns of gene expression.

Transsynaptic mapping of the postsynaptic connections of mushroom body output neurons reveal both divergent and convergent projections allowing for multimodal integration prior to initiation of an output response.

Sex-specific characteristics of the fruit fly courtship behavior are not specified by a single binary switch, but as a combination of traits that are modularly specified by separable genetic switches.

Male-type aggressive and courtship behaviors of the fruit flies are differentially specified by two sex-determining genes, providing a substrate for the evolution to sculpt these two behaviors independently.

Lineally related neurons in the adult Drosophila ventral nerve cord use the same neurotransmitter to communicate with other neurons.

Building on previous work (Aso et al., 2014a; Aso et al., 2014b), cell-type-specific drivers and optogenetics are used to control the local release of dopamine and reveal that distinct learning rules are implemented in parallel memory units.

A library of a thousand gene-specific intronic GAL4 insertions was generated, which caused a severe loss of function, permitted the assessing of gene expression, allowed the rescue of mutated gene with UAS-cDNA constructs, and provided a tool for the reversion of mutant phenotypes in specific cells or tissues.