Metabolomics and stable isotope labelling studies of virulent Mycobacterium tuberculosis reveal a de-centralised metabolic network able to utilise various amino acids as nitrogen sources to a better extent than ammonium.

By performing ¹⁵N pulse-labeling of mice, the turnover of hundreds of proteins in eye tissues was measured by mass spectrometry that revealed long-lived metabolic enzymes in the lens.

Stable isotope labeling reveals that NAD crosses the inner membrane of mammalian mitochondria via an unknown transporter.

Mitochondrial inner membrane translocation of presequence-containing proteins by the single bifunctional TIM complex of T. brucei requires an non-canonical J domain-containing protein.

Although puromycin staining is often used to examine subcellular translation, puromycin-labeled proteins are rapidly released from ribosomes even in the presence of elongation inhibitors, which may confound translation site localization.

Mitochondrial fusion enables a metabolic transition during spermatogenesis.

Cell-based high-throughput screening identifies IBT21 as a chemical chaperone, that inhibits ER protein aggregation and prevents the cell death caused by a proteotoxin, the aggregation-prone prion protein.

Lactate in pancreatic cancer micro-environment can lead to TET enzyme activation and loss of DNA methylation seen in cancer associated fibroblasts.

In a developing yeast colony, cells go from homogeneous states to spatially organized, specialized metabolic states, and the new metabolic states depend on resources produced by the original state.

Dispensable loops shield the functionally-important extracellular loops of the essential Gram-negative bacterial outer membrane protein LptD from antibody interference.