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    1. Ecology

    Algal-fungal symbiosis leads to photosynthetic mycelium

    Zhi-Yan Du, Krzysztof Zienkiewicz ... Gregory M Bonito
    The capacity for symbiosis between photosynthetic microalgae and early diverging lineages fungi was demonstrated with microscopy and stable isotope exchange of carbon and nitrogen.
    1. Biochemistry and Chemical Biology
    2. Evolutionary Biology

    'Palaeoshellomics’ reveals the use of freshwater mother-of-pearl in prehistory

    Jorune Sakalauskaite, Søren H Andersen ... Beatrice Demarchi
    Ancient proteomes from tiny shell ornaments were successfully characterised for the first time, showing the unexpected use of mother-of-pearl from local riverine molluscs in both coastal and inland prehistoric sites.
    1. Ecology

    Using aquatic animals as partners to increase yield and maintain soil nitrogen in the paddy ecosystems

    Liang Guo, Lufeng Zhao ... Xin Chen
    Aquatic animals cocultured with rice in paddy ecosystems can increase food production, improve nitrogen (N)-use efficiency, and maintain soil fertility by reducing weeds, and promoting recycle and complementary use of N.
    1. Cell Biology

    Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling

    Yoanna Ariosa-Morejon, Alberto Santos ... Tonia L Vincent
    Protein dynamics signatures in ageing collagen-rich tissues that are central to frequent age-related diseases such as osteoporosis and osteoarthritis.
    1. Ecology

    Cyclic bouts of extreme bradycardia counteract the high metabolism of frugivorous bats

    M Teague O'Mara, Martin Wikelski ... Dina KN Dechmann
    Novel heart rate strategies that minimize energy expenditure during the day are necessary to cope with high energy nocturnal lifestyles of tent-making bats.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    MYC overrides HIF-1α to regulate proliferating primary cell metabolism in hypoxia

    Courtney A Copeland, Benjamin A Olenchock ... William M Oldham
    Hypoxia does not increase glycolysis in proliferating primary cells and antagonizes the increase in glycolysis caused by activation of hypoxia-inducible factor in normoxia, in part, through activation of MYC signaling pathways.
    1. Microbiology and Infectious Disease

    Heavy isotope labeling and mass spectrometry reveal unexpected remodeling of bacterial cell wall expansion in response to drugs

    Heiner Atze, Yucheng Liang ... Michel Arthur
    Labeling with 13C and 15N in the absence of metabolic engineering enabled the exploration of peptidoglycan metabolism at a very fine level of detail based on kinetic characterization of isotopologues predicted to occur according to known recycling and biosynthesis pathways.
    1. Biochemistry and Chemical Biology

    Long-lived metabolic enzymes in the crystalline lens identified by pulse-labeling of mice and mass spectrometry

    Pan Liu, Seby Louis Edassery ... Jing Jin
    By performing 15N pulse-labeling of mice, the turnover of hundreds of proteins in eye tissues was measured by mass spectrometry that revealed long-lived metabolic enzymes in the lens.
    1. Structural Biology and Molecular Biophysics

    Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states

    Dan Tan, Qiang Li ... Xiaoguang Lei
    An enrichable cross-linker with optional isotope labeling quadruples the number of cross-linked peptides identified from high-complexity samples, enhancing proteome-wide analysis of protein-protein interactions and protein conformational changes by mass spectrometry.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Single-cell RNA-seq reveals transcriptomic heterogeneity mediated by host–pathogen dynamics in lymphoblastoid cell lines

    Elliott D SoRelle, Joanne Dai ... Micah A Luftig
    Single-cell RNA sequencing highlights the influence of host–pathogen interactions and stochasticity on transcriptional and phenotypic variance in lymphoblastoid cell lines derived from Epstein–Barr virus-infected primary B cells.