The glucose-sensing transcription factor MondoA regulates zebrafish epiboly via cholesterol biosynthesis genes including the human disease gene Nsdhl, revealing an unknown role for metabolic glucose signalling in vertebrate development.

Parallel branches of the ERAD pathway regulate sterol synthesis at multiple steps via feedback inhibition.

Trypanosoma cruzi intracellular amastigotes exhibit rapid resistance to azoles, independent of genetic selection, which is dependent on metabolic state and mechanistically distinct from latency.

Cells use two interdigitated biosynthetic pathways that can be regulated independently to produce diverse bioactive sterols.

The retrograde vesicular trafficking GARP complex, which is mutated in a neurodegenerative disease, is important for sphingolipid homeostasis in yeast and mammalian cells.

CRISPR/Cas9 genome-wide screens using sterol-sensitive endogenous HMG-CoA reductase (HMGCR) reporter identify the sterol-responsive RNF145 and gp78 as independently responsible for sterol-accelerated degradation of HMGCR, the rate-limiting enzyme of cholesterol biosynthesis.

The patched hedgehog receptor inhibits the transmembrane transducer smoothened by reducing the accessibility of cholesterol locally at the membrane of the primary cilium.

SREBP-2 directly regulates genes involved in cholesterol homeostasis and indirectly regulates fatty acid synthesis through the production of a ligand responsible for the activation of LXR and SREBP-1c.

The E3 ubiquitin ligase RNF145 is shown to contribute to crosstalk between the LXR and SREBP-2 pathways in the regulation of cholesterol homeostasis.

Genetic and biochemical analyses, coupled with fluorescence imaging, reveal that spatial compartmentalization of HMG-CoA Reductases at yeast ER-lysosome contacts modulates mevalonate pathway flux during glucose restriction, enabling metabolic adaptation.