Rigorous biochemical and structural analyses reveal the precise topology of cohesin's association with DNA and suggest a mechanism for how DNA is transported inside the ring.
Clara Bouyx, Marion Schiavone ... Jean Marie François
The role of amyloid-β-aggregation sequence and of the various domains in the physiological function of the FLO11-encoded adhesin in the yeast Saccharomyces cerevisiae are disclosed in this report.
Client protein-driven reversal of endoplasmic reticulum chaperone (BiP) mediated-repression is revealed as a principal component of the regulation of the unfolded protein response transducer IRE1 in cells.
A high-throughput method to profile tyrosine kinases and phosphotyrosine recognition domains reveals new rules for sequence specificity and maps the effects of mutations on the recognition of tyrosine phosphorylation sites.
A time-resolved analysis of protein and RNA concentrations and interactions during proteostasis stress highlights the dominant role of translation regulation and a shift of energy metabolism.
The development of an integrative computational-experimental approach for identifying small molecules that can disrupt RNA:protein interactions in vitro and in cells led to the identification of several inhibitors of YBX1 in the micromolar range, including a previously approved drug.
Development of a live single-molecule imaging approach to visualize XBP1 mRNAs, which are recruited for translation on the ER and efficiently spliced in the absence of large IRE1α foci.
Jeffrey R Moffitt, Shristi Pandey ... Xiaowei Zhuang
Transcriptome-scale RNA imaging and lifetime measurements reveal that the E. coli transcriptome is spatially organized and that this organization modulates the post-transcriptional fate of bacterial mRNAs.
