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    1. Plant Biology
    2. Structural Biology and Molecular Biophysics

    In situ cryo-ET structure of phycobilisome–photosystem II supercomplex from red alga

    Meijing Li, Jianfei Ma ... Sen-Fang Sui
    The nature of the phycobilisome–photosystem II supercomplex on the native thylakoid determined with cryo-electron tomography at an unprecedented resolution reveals that one phycobilisome interconnects with six photosystem monomers.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Molecular and topological reorganizations in mitochondrial architecture interplay during Bax-mediated steps of apoptosis

    Nicholas R Ader, Patrick C Hoffmann ... Wanda Kukulski
    Correlative microscopy and electron cryo-tomography on apoptotic HeLa cells reveal remodeling of outer and inner mitochondrial membranes, Bax cluster ultrastructure and ATP synthase reorganization in ruptured mitochondria.
    1. Structural Biology and Molecular Biophysics

    Structure and in situ organisation of the Pyrococcus furiosus archaellum machinery

    Bertram Daum, Janet Vonck ... Werner Kühlbrandt
    Using both electron cryo-tomography and helical reconstruction, the first structure of the entire archaellum machinery with an assembled filament has been determined, providing the structural basis for our understanding of archaeal motility.
    1. Cell Biology
    2. Physics of Living Systems

    Correlative all-optical quantification of mass density and mechanics of subcellular compartments with fluorescence specificity

    Raimund Schlüßler, Kyoohyun Kim ... Jochen Guck
    The combination of optical diffraction tomography and Brillouin microscopy in a single setup enables to quantitatively map the viscoelastic properties of cellular compartments such as aggregates and stress granules in vivo.
    1. Microbiology and Infectious Disease

    The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching

    Brittany L Carroll, Tatsuro Nishikino ... Jun Liu
    In situ structures of the flagellar motors genetically docked in CCW and CW rotational states reveal major rearrangement that facilitates rotational switch in Vibrio alginolyticus..
    1. Cell Biology

    The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle

    Faye M Nixon, Cristina Gutiérrez-Caballero ... Stephen J Royle
    Microtubules of mitotic spindle fibers are positioned and held together by a meshwork of multipolar connectors.
    1. Cell Biology

    Cardiac ryanodine receptor distribution is dynamic and changed by auxiliary proteins and post-translational modification

    Parisa Asghari, David RL Scriven ... Edwin DW Moore
    Repositioning the type II ryanodine receptors on the sarcoplasmic reticulum membrane is a potential new mechanism regulating their function, and therefore the strength of cardiac contraction.
    1. Structural Biology and Molecular Biophysics
    2. Microbiology and Infectious Disease

    In situ structural analysis of the Yersinia enterocolitica injectisome

    Mikhail Kudryashev, Marco Stenta ... Henning Stahlberg
    The basal body of the type-III secretion system of Yersinia enterocolitica within bacterial membranes shows elasticity and is longer than related isolated systems.
    1. Structural Biology and Molecular Biophysics

    The molecular infrastructure of glutamatergic synapses in the mammalian forebrain

    Julia Peukes, Charlie Lovatt ... René A Frank
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Cell Biology
    2. Neuroscience

    Optogenetics and electron tomography for structure-function analysis of cochlear ribbon synapses

    Rituparna Chakrabarti, Lina María Jaime Tobón ... Carolin Wichmann
    Ultrastructural analysis of optogenetically stimulated inner hair cell ribbon synapses reveals normally sized synaptic vesicles and enhanced vesicle docking at the active zone membrane.