2,782 results found
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers

    Marijn de Boer et al.
    Selective import via bacterial ABC importers is facilitated by a hitherto unrecognized complexity in the conformational dynamics of the substrate-binding proteins.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structure and substrate ion binding in the sodium/proton antiporter PaNhaP

    David Wöhlert et al.
    The X-ray structure of the PaNhaP provides a unique view of substrate-ion binding and release in electroneutral sodium/proton antiporters.
    1. Biochemistry and Chemical Biology

    Mia40 is a trans-site receptor that drives protein import into the mitochondrial intermembrane space by hydrophobic substrate binding

    Valentina Peleh et al.
    The import of proteins into the intermembrane space of mitochondria is driven by binding to the trans-site receptor Mia40 and not by the oxidation of cysteine residues.
    1. Structural Biology and Molecular Biophysics

    Crystal structure of the full Swi2/Snf2 remodeler Mot1 in the resting state

    Agata Butryn et al.
    The structure of the entire Mot1 Swi2/Snf2 protein uncovers an unexpected auto-inhibited resting state activated by substrate binding, and suggests a DNA pulling mechanism of TBP displacement.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Interactions between a subset of substrate side chains and AAA+ motor pore loops determine grip during protein unfolding

    Tristan A Bell et al.
    The AAA+ protein unfolding motor ClpX grips substrates with the uppermost part of its substrate-binding pore, and requires interactions with hydrophobic amino acid side chains to operate with optimal efficiency.
    1. Structural Biology and Molecular Biophysics

    Promiscuous binding by Hsp70 results in conformational heterogeneity and fuzzy chaperone-substrate ensembles

    Rina Rosenzweig et al.
    Multiple chaperone binding sites on substrates suggest a mechanism by which the Hsp70 chaperone can circumvent kinetic traps in protein folding.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Down-regulation of TORC2-Ypk1 signaling promotes MAPK-independent survival under hyperosmotic stress

    Alexander Muir et al.
    Building on previous work (Muir et al., 2014), we validate the utility of a screen for new protein kinase substrates and define the underlying molecular basis of a new mechanism for responding to hypertonic conditions.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    In vitro FRET analysis of IRE1 and BiP association and dissociation upon endoplasmic reticulum stress

    Megan C Kopp et al.
    Quantitative FRET UPR induction assay is used to measure IRE1 and BIP association and dissociation by a variety of ER misfolded proteins and by an important BiP substrate-binding domain mutant, significantly enhancing the evidence for the allosteric UPR induction model.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    AMPylation targets the rate-limiting step of BiP’s ATPase cycle for its functional inactivation

    Steffen Preissler et al.
    AMPylation of BiP allosterically interferes with stimulation of its ATPase activity by J-proteins that is required for high affinity substrate binding.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    The AAA ATPase Vps4 binds ESCRT-III substrates through a repeating array of dipeptide-binding pockets

    Han Han et al.
    A 3.2 Å resolution structure of Vps4 provides a detailed model for protein substrate binding and translocation by AAA ATPases.

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