John H Day, Catherine M Della Santina ... Laurie A Boyer
A simple and accessible method for high-throughput super-resolution fluorescence microscopy that is compatible with commonly used staining practices, 96-well cell culture plates, and confocal microscopes.
Super-resolution microscopy, biochemical and functional analyses reveal how α-taxilin and γ-taxilin are assembled at the subdistal appendages and their roles in microtubule organization.
Romain F Laine, Gemma Goodfellow ... Clemens F Kaminski
Machine learning in conjunction with super-resolution imaging allows for the first time to quantitatively analyse large and heterogenous virus samples structure at a high throughput and specificity.
Yasel Garcés Suárez, Jose L Martínez ... Carlos F Arias
Super-resolution microscopy reveals, at nanometric-scale, the highly organized protein structure of viroplasms, the viral factories used by rotavirus to replicate its genome and assemble new viral particles.
Nuclear pores assemble asymmetrically, by an inside-out evagination of the inner nuclear membrane that grows in diameter and depth until it fuses with the flat outer nuclear membrane.
Thomas Pfeiffer, Stefanie Poll ... U Valentin Nägerl
Super-resolution STED microscopy is demonstrated for the first time in the deeply embedded mouse hippocampus in vivo, revealing direct evidence for an unprecedentedly high level of synapse remodeling in a brain structure closely associated with memory processing.
Ando Christian Zehrer, Ana Martin-Villalba ... Helge Ewers
High-quality fluorescence live-cell and single molecule imaging via computer-controlled, user-assembled microscope that fits in incubator, can in large parts be 3D printed and employs open source software and electronics.
Molecular beacon based FISH shows the capability of visualizing a 2.5 kb non-repetitive genomic DNA sequence in situ in human or mouse nuclear genome at super resolution.
Neuroligin 1 is a critical adhesion molecule which organizes AMPA receptor nanodomains in close vicinity to pre-synaptic release sites, and whose genetic or chemical disruption severely impairs synaptic transmission properties.