1,829 results found
    1. Cell Biology

    High-throughput expansion microscopy enables scalable super-resolution imaging

    John H Day, Catherine M Della Santina ... Laurie A Boyer
    A simple and accessible method for high-throughput super-resolution fluorescence microscopy that is compatible with commonly used staining practices, 96-well cell culture plates, and confocal microscopes.
    1. Cell Biology

    α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization

    Dandan Ma, Fulin Wang ... Jianguo Chen
    Super-resolution microscopy, biochemical and functional analyses reveal how α-taxilin and γ-taxilin are assembled at the subdistal appendages and their roles in microtubule organization.
    1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Structured illumination microscopy combined with machine learning enables the high throughput analysis and classification of virus structure

    Romain F Laine, Gemma Goodfellow ... Clemens F Kaminski
    Machine learning in conjunction with super-resolution imaging allows for the first time to quantitatively analyse large and heterogenous virus samples structure at a high throughput and specificity.
    1. Microbiology and Infectious Disease

    Nanoscale organization of rotavirus replication machineries

    Yasel Garcés Suárez, Jose L Martínez ... Carlos F Arias
    Super-resolution microscopy reveals, at nanometric-scale, the highly organized protein structure of viroplasms, the viral factories used by rotavirus to replicate its genome and assemble new viral particles.
  1. Nuclear pore assembly proceeds by an inside-out extrusion of the nuclear envelope

    Shotaro Otsuka, Khanh Huy Bui ... Jan Ellenberg
    Nuclear pores assemble asymmetrically, by an inside-out evagination of the inner nuclear membrane that grows in diameter and depth until it fuses with the flat outer nuclear membrane.
    1. Neuroscience

    Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo

    Thomas Pfeiffer, Stefanie Poll ... U Valentin Nägerl
    Super-resolution STED microscopy is demonstrated for the first time in the deeply embedded mouse hippocampus in vivo, revealing direct evidence for an unprecedentedly high level of synapse remodeling in a brain structure closely associated with memory processing.
    1. Neuroscience

    Gap junction-mediated glycinergic inhibition ensures precise temporal patterning in vocal behavior

    Boris P Chagnaud, Jonathan T Perelmuter ... Andrew H Bass
    Inhibition provides a means for achieving temporal precision for rapid modulation of an acoustic signal in a vertebrate vocal network.
    1. Cell Biology

    An open-source, high-resolution, automated fluorescence microscope

    Ando Christian Zehrer, Ana Martin-Villalba ... Helge Ewers
    High-quality fluorescence live-cell and single molecule imaging via computer-controlled, user-assembled microscope that fits in incubator, can in large parts be 3D printed and employs open source software and electronics.
    1. Chromosomes and Gene Expression

    Super-resolution imaging of a 2.5 kb non-repetitive DNA in situ in the nuclear genome using molecular beacon probes

    Yanxiang Ni, Bo Cao ... Hanben Niu
    Molecular beacon based FISH shows the capability of visualizing a 2.5 kb non-repetitive genomic DNA sequence in situ in human or mouse nuclear genome at super resolution.
    1. Neuroscience

    Pre-post synaptic alignment through neuroligin-1 tunes synaptic transmission efficiency

    Kalina T Haas, Benjamin Compans ... Eric Hosy
    Neuroligin 1 is a critical adhesion molecule which organizes AMPA receptor nanodomains in close vicinity to pre-synaptic release sites, and whose genetic or chemical disruption severely impairs synaptic transmission properties.

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