A combination of imaging experiments and computer simulations on a model lipid membrane integrate the 'picket fence' and 'raft' models, and suggest that the interplay between actin binding, lipid phase separation and curvature compartmentalize the membrane.
Connor J Thompson, Zhaoqian Su ... Daniel K Schwartz
A combined experimental and computational approach was developed to understand lateral interactions between membrane-bound proteins and used to quantify the contributions of specific and non-specific interactions to cadherin cis-binding kinetics.
Biochemical studies in combination with computational modeling and molecular dynamics simulations reveal that the lipid bilayer promotes intramembrane proteolysis by stabilizing the enzyme-substrate complex and the protease active site.
Víctor M Hernández-Rocamora, Natalia Baranova ... Waldemar Vollmer
The synthesis of bacterial cell wall peptidoglycan was reconstituted in lipid bilayers and detected by a novel Förster resonance energy transfer real-time assay.
A new and general mechanism describes the organization of membrane proteins and their cytoplasmic ligands into micrometer-scale clusters, based on polymerization and concomitant phase separation of multivalent proteins.
A high-resolution method to quantify interactions between lipid bilayers and single proteins under controlled load is presented and applied to key proteins involved in membrane fusion and formation and maintenance of membrane contact sites.
Sven Kenjiro Vogel, Ferdinand Greiss ... Petra Schwille
Building on previous work (Vogel et al., 2013b), it is shown that myosin-driven actin filament rearrangements actively move, split or fuse individual lipid domains and change their overall shape.
Lindsay B Case, Milagros De Pasquale ... Michael K Rosen
Biochemical and cell biological data suggest a model of nascent integrin adhesion complex formation based on synergistic phase separation of pathways surrounding the focal adhesion kinase (FAK) and the adaptor protein p130Cas.
The axial organization and dynamics of the HOPS complex at membrane surface are resolved by graphene-induced energy transfer with subnanometer resolution.
