By integrating multiplexed error-robust fluorescence in situ hybridization (MERFISH), deep learning, optical sectioning, and protocol optimization, thick-tissue 3D MERFISH allows volumetric single-cell transcriptome imaging of thick-tissue samples, broadening the scope of applications of spatial genomics.

Astrocytic ER calcium release and gap junction communication exhibit circadian modulation driven by HERPUD1, highlighting the importance of astrocytic circadian rhythms in brain function.

Discovery of critical brain nodes explaining how sevoflurane-induced general anesthesia works and how the post-anesthesia stress responses appear.

Blood-borne factors contribute to changes in the circadian transcriptome with age.

Distinct local dynamics of different hypothalamus areas were detected in response to changing illuminance and could contribute to light’s impact on cognition.

Preoptic neurotensinergic neurons co-release GABA and neurotensin, triggering a rapid initial inhibition followed by a sustained excitation, and mice lacking GABA release from these neurons exhibit altered thermoregulation.