Biophysical and functional data strongly support the notion that Munc18-1 acts as a template to assemble the neuronal SNARE complex, and that inhibition of this activity underlies diverse forms of regulation of neurotransmitter release.
Isothermal titration calorimetry experiments clarify apparently discrepant results described previously and show that N-terminal sequences of complexin bind to SNARE complexes containing C-terminally truncated synaptobrevin when they include the syntaxin-1 juxtamembrane region.
The organization of layered/laminated axon projections in specific regions of the fruitfly central brain is regulated by short-range repulsive guidance and is critical for local inhibitory circuit formation and function.
Global phosphoproteomic analysis in nerve terminal during exocytosis reveals 252 uniquely regulated phosphosites, highlighting complex regulation of active zone proteins at multiple sites and the role of specific kinases/phosphatases.
Analyzing a map of all cholinergic neurons in the nematode C. elegans shows how these neurons are embedded in the C. elegans connectome and reveals the regulatory factors controlling their development.