In transgenesis assays involving diploid model organisms, two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs allow the systematic creation of homozygous transgenic lines and minimize the number of wasted animals.
Yeast promoters can harbor multiple natural DNA variants that influence gene expression, interact genetically, evolve under negative selection, alter transcription factor motifs, and remain challenging to predict.
While antimicrobial cocktails are highly effective for defence against pathogenic microbes, the innate immune response may instead employ highly specific peptidic antibiotics to combat certain natural enemies.
The metalloprotease ADAM10 modulates axon and synapse function by cleaving numerous synaptic and axonal membrane proteins in the central nervous system.
A new approach combines guide RNA multiplexing with CRISPR activation and interference to facilitate functional studies of transposable elements present in hundreds of copies throughout the human genome.
Compiling public datasets into a single, centralised repository and linking directly to analytical software completely transforms the scale and scope of causal inference across the phenome.
