Mark Hedglin, Senthil K Perumal ... Stephen Benkovic
Fluorescence resonance energy transfer has been used to explore the interactions between DNA polymerases, sliding clamps and clamp loaders as DNA is replicated in human cells.
The recycling of transmitter vesicles at synapses shows calcium-dependent regulation that depends on the phosphorylation status of an enzyme called dynamin.
Optogenetics has revealed that synaptic vesicles can be recycled extremely rapidly in nematodes, indicating that existing models for how synapses 'reload' may need to be revised.
The precise position of UNC-13 at the active zone near a synapse depends on the N-terminus of the protein, and the C2A domain in particular, and is essential for accelerating neurotransmitter release.
Associative learning, but not passive odorant exposure, induces a novel long lasting functional plasticity in the periphery of mouse olfactory system, making previously encountered odors easier to detect in the future.
Alexander M Jones, Jonas ÅH Danielson ... Wolf B Frommer
Fluorescent sensors for the hormone abscisic acid have been developed using a high-throughput platform, and used to monitor hormone dynamics in plant roots and leaves.
HIV-1 viral protein u (Vpu) can stimulate novel versions of canonical interactions with the clathrin adaptor AP1 to counteract the host antiviral protein BST2.
Braden T Lobingier, Daniel P Nickerson ... Alexey J Merz
Sec1/Munc18 (SM) proteins shield SNARE complexes from NSF/Sec18-mediated disassembly through cooperative binding interactions with SNARE complexes and the universal co-chaperone α-SNAP/Sec17.