55 results found
    1. Plant Biology
    2. Structural Biology and Molecular Biophysics

    Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision

    Wojciech Wietrzynski, Miroslava Schaffer ... Benjamin D Engel
    In situ cryo-electron tomography resolves individual photosynthetic complexes within native thylakoid membranes, revealing how thylakoid architecture sorts proteins into discrete membrane domains.
    1. Plant Biology
    2. Structural Biology and Molecular Biophysics

    Structure of Dunaliella photosystem II reveals conformational flexibility of stacked and unstacked supercomplexes

    Ido Caspy, Maria Fadeeva ... Nathan Nelson
    Photosystem II, the pigment-protein complex responsible for water oxidation in photosynthesis, was found to exist in two different core conformations with altered antennae connectivity.
    1. Plant Biology

    A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis

    Rosa Pipitone, Simona Eicke ... Emilie Demarsy
    Serial-Block-Face Scanning Electron Microscopy (SBF-SEM) associated with biomolecular analysis show that chloroplast differentiation proceeds by distinct ‘structure establishment’ and ‘chloroplast proliferation’ phases, each with differential protein and lipid regulation.
    1. Structural Biology and Molecular Biophysics
    2. Plant Biology

    Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography

    Benjamin D Engel, Miroslava Schaffer ... Wolfgang Baumeister
    The first 3D views of the native algal chloroplast provide new insights into thylakoid biogenesis, photosynthesis, and carbon fixation.
    1. Plant Biology
    2. Structural Biology and Molecular Biophysics

    In situ cryo-ET structure of phycobilisome–photosystem II supercomplex from red alga

    Meijing Li, Jianfei Ma ... Sen-Fang Sui
    The nature of the phycobilisome–photosystem II supercomplex on the native thylakoid determined with cryo-electron tomography at an unprecedented resolution reveals that one phycobilisome interconnects with six photosystem monomers.
    1. Plant Biology

    Regulation of photosynthetic electron flow on dark to light transition by ferredoxin:NADP(H) oxidoreductase interactions

    Manuela Kramer, Melvin Rodriguez-Heredia ... Guy Thomas Hanke
    The regulatory switch from protection to assimilation, which plants use to exploit natural, fluctuating light, involves movement of the enzyme ferredoxin:NADP(H) oxidoreductase between chloroplast membrane complexes.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Assembling the Tat protein translocase

    Felicity Alcock, Phillip J Stansfeld ... Ben C Berks
    Evolutionary bioinformatics and experimentation are applied to the components of the Tat protein transport system to elucidate the structure of the membrane-bound receptor complex and to deduce a molecular description for its substrate-triggered activation.
    1. Plant Biology
    2. Structural Biology and Molecular Biophysics

    Identification of distinct pH- and zeaxanthin-dependent quenching in LHCSR3 from Chlamydomonas reinhardtii

    Julianne M Troiano, Federico Perozeni ... Gabriela S Schlau-Cohen
    Light-harvesting complex stress-related is a protein from photosynthetic green algae that prevents damage from sunlight via two distinct conformational processes, which protect against different timescales of solar fluctuations.
    1. Cell Biology
    2. Plant Biology

    Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts

    Masanori Izumi, Sakuya Nakamura ... Shinya Hagihara
    A plant autophagy pathway transports chloroplast stroma and envelope components into the vacuole through the division and encapsulation of the site of chloroplasts that is associated with developing autophagosome.
    1. Plant Biology

    Characterization of tryptophan oxidation affecting D1 degradation by FtsH in the photosystem II quality control of chloroplasts

    Yusuke Kato, Hiroshi Kuroda ... Wataru Sakamoto
    Oxidative modification of Tryptophan residues in the reaction center protein D1 may be a key to drive the Photosystem II repair, likely enhancing accessibility of FtsH protease to D1.

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