Developing T cells whose TCRs have relatively low reactivity experience very brief TCR signaling events, experience delayed positive selection, and retaina preselection gene expression signature as they mature.

Deficiency of capicua, a transcription factor that suppresses autoimmunity, impairs positive and negative selection processes by attenuating TCR signaling in CD4⁺CD8⁺ double-positive thymocytes.

Sequence signatures can be used to discriminate between selected and non-selected immune repertoires at different stages only at the collective population level but not at the level of single cells.

The thymic selection of the human T-cell receptor repertoire releases polyspecific receptors with the ability to recognize and respond to peptides from unrelated viruses.

The development of Natural Killer T Cells is controlled by Histone Deacetylase 7, a function that combined with its known role in thymic negative selection provides a potential mechanism explaining its association with tissue-specific autoimmunity in humans.

Self-reactive thymocyte death is most efficient when peptide-presenting cells are phagocytic, and blocking phagocytosis inhibits self-reactive thymocyte death, suggesting that phagocytes play central roles during thymocyte negative selection.

Two-photon microscopy, combined with chemotaxis assays, synchronized thymocyte selection studies, and flow cytometry analyses, reveal that CCR4 and CCR7 promote medullary entry and central tolerance of immature and mature post-positive selection thymocyte subsets, respectively, with distinct outcomes for central tolerance.

Fam49b is a critical regulator of selection process to ensure normal thymocyte development and peripheral T cells survival.

A novel zinc finger transcription factor regulates expression of multiple genes in late stage thymocytes and recent thymic emigrants to promote formation of the naïve T cell compartment.

Thymus ageing is characterised by both compositional and transcriptional shifts amongst epithelial cells that perturb their differentiation, contribute to organ atrophy and ultimately impair immune function.