5,735 results found
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    An improved fluorescent noncanonical amino acid for measuring conformational distributions using time-resolved transition metal ion FRET

    William N Zagotta et al.
    Time-resolved transition metal ion fluorescence resonance energy transfer using a fluorescent, noncanonical amino acid donor and metal ion acceptor measures distances and distance distributions in proteins, allowing the equilibrium distributions among conformational states to be accurately determined.
    1. Developmental Biology

    Feedback regulation of cytoneme-mediated transport shapes a tissue-specific FGF morphogen gradient

    Lijuan Du et al.
    FGF morphogen gradient self-generates a recipient tissue-specific contour by feedback-regulating its cytoneme-mediated dispersion.
    1. Cell Biology
    2. Computational and Systems Biology

    Removing physiological motion from intravital and clinical functional imaging data

    Sean C Warren et al.
    Image-based motion correction enables the use of fluorescence lifetime and other functional imaging modalities in an intravital and clinical context in the presence of physiological motion.
    1. Developmental Biology

    A gradient of Wnt activity positions the neurosensory domains of the inner ear

    Magdalena Żak, Nicolas Daudet
    Manipulations of Wnt signalling in the chicken embryo reveal how it controls in a dose-dependent manner the formation of the neurosensory territories of the early developing inner ear.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Monitoring site-specific conformational changes in real-time reveals a misfolding mechanism of the prion protein

    Ishita Sengupta, Jayant Udgaonkar
    Multi-site FRET measurements of moPrP oligomerization at low pH indicate that major conformational changes take place as monomers reversibly transform into large oligomers, which subsequently disassemble reversibly into small oligomers.
    1. Neuroscience
    2. Physics of Living Systems

    The neuronal calcium sensor Synaptotagmin-1 and SNARE proteins cooperate to dilate fusion pores

    Zhenyong Wu et al.
    During neurotransmitter release, calcium-induced membrane insertion of the C2B domain of Synaptotagmin re-orients the bound SNARE complex which dilates the fusion pore in a mechanical lever action.
    1. Chromosomes and Gene Expression

    Noncoding RNA-nucleated heterochromatin spreading is intrinsically labile and requires accessory elements for epigenetic stability

    R A Greenstein et al.
    Heterochromatin spreading in fission yeast predominantly produces intergenerationally unstable outcomes, requiring an accessory element that represses histone turnover.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Assembly and positioning of actomyosin rings by contractility and planar cell polarity

    Ivonne M Sehring et al.
    Contractility wins over the polarity pathway in a tug-of-war to position a cytokinesis-like actomyosin ring at the equator of notochord cells.
    1. Structural Biology and Molecular Biophysics

    A bend, flip and trap mechanism for transposon integration

    Elizabeth R Morris et al.
    Structural and biochemical analyses of a eukaryotic DNA transpososome in the integration step reveal how mariner/Tc1 transposons are selectively integrated into a TA target sequence.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Transient kinetic studies of the antiviral Drosophila Dicer-2 reveal roles of ATP in self–nonself discrimination

    Raushan K Singh et al.
    A real-time, stopped-flow method, in combination with protein-induced fluorescence enhancement, Förster resonance energy transfer, and time-resolved fluorescence spectroscopy, reveals a kinetic framework for understanding Dicer as a complex molecular motor.

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